diff --git a/python/pyspark/sql/tests/test_functions.py b/python/pyspark/sql/tests/test_functions.py
index 8e01f5d170ff5..2fde3edc2486c 100644
--- a/python/pyspark/sql/tests/test_functions.py
+++ b/python/pyspark/sql/tests/test_functions.py
@@ -81,7 +81,10 @@ def test_function_parity(self):
         missing_in_py = jvm_fn_set.difference(py_fn_set)
 
         # Functions that we expect to be missing in python until they are added to pyspark
-        expected_missing_in_py = set()
+        expected_missing_in_py = set(
+            # TODO(SPARK-53108): Implement the time_diff function in Python
+            ["time_diff"]
+        )
 
         self.assertEqual(
             expected_missing_in_py, missing_in_py, "Missing functions in pyspark not as expected"
diff --git a/sql/api/src/main/scala/org/apache/spark/sql/functions.scala b/sql/api/src/main/scala/org/apache/spark/sql/functions.scala
index 49fa45ed02cbe..95ca5b44bf023 100644
--- a/sql/api/src/main/scala/org/apache/spark/sql/functions.scala
+++ b/sql/api/src/main/scala/org/apache/spark/sql/functions.scala
@@ -6292,6 +6292,28 @@ object functions {
   def timestamp_add(unit: String, quantity: Column, ts: Column): Column =
     Column.internalFn("timestampadd", lit(unit), quantity, ts)
 
+  /**
+   * Returns the difference between two times, measured in specified units. Throws a
+   * SparkIllegalArgumentException, in case the specified unit is not supported.
+   *
+   * @param unit
+   *   A STRING representing the unit of the time difference. Supported units are: "HOUR",
+   *   "MINUTE", "SECOND", "MILLISECOND", and "MICROSECOND". The unit is case-insensitive.
+   * @param start
+   *   A starting TIME.
+   * @param end
+   *   An ending TIME.
+   * @return
+   *   The difference between `end` and `start` times, measured in specified units.
+   * @note
+   *   If any of the inputs is `NULL`, the result is `NULL`.
+   * @group datetime_funcs
+   * @since 4.1.0
+   */
+  def time_diff(unit: Column, start: Column, end: Column): Column = {
+    Column.fn("time_diff", unit, start, end)
+  }
+
   /**
    * Returns `time` truncated to the `unit`.
    *
diff --git a/sql/core/src/test/scala/org/apache/spark/sql/TimeFunctionsSuiteBase.scala b/sql/core/src/test/scala/org/apache/spark/sql/TimeFunctionsSuiteBase.scala
index 005bfcb13d2e8..556755a73ff7c 100644
--- a/sql/core/src/test/scala/org/apache/spark/sql/TimeFunctionsSuiteBase.scala
+++ b/sql/core/src/test/scala/org/apache/spark/sql/TimeFunctionsSuiteBase.scala
@@ -241,6 +241,75 @@ abstract class TimeFunctionsSuiteBase extends QueryTest with SharedSparkSession
     checkAnswer(result2, expected)
   }
 
+  test("SPARK-53108: time_diff function") {
+    // Input data for the function.
+    val schema = StructType(Seq(
+      StructField("unit", StringType, nullable = false),
+      StructField("start", TimeType(), nullable = false),
+      StructField("end", TimeType(), nullable = false)
+    ))
+    val data = Seq(
+      Row("HOUR", LocalTime.parse("20:30:29"), LocalTime.parse("21:30:28")),
+      Row("second", LocalTime.parse("09:32:05.359123"), LocalTime.parse("17:23:49.906152")),
+      Row("MicroSecond", LocalTime.parse("09:32:05.359123"), LocalTime.parse("17:23:49.906152"))
+    )
+    val df = spark.createDataFrame(spark.sparkContext.parallelize(data), schema)
+
+    // Test the function using both `selectExpr` and `select`.
+    val result1 = df.selectExpr(
+      "time_diff(unit, start, end)"
+    )
+    val result2 = df.select(
+      time_diff(col("unit"), col("start"), col("end"))
+    )
+    // Check that both methods produce the same result.
+    checkAnswer(result1, result2)
+
+    // Expected output of the function.
+    val expected = Seq(
+      0,
+      28304,
+      28304547029L
+    ).toDF("diff").select(col("diff"))
+    // Check that the results match the expected output.
+    checkAnswer(result1, expected)
+    checkAnswer(result2, expected)
+
+    // NULL result is returned for any NULL input.
+    val nullInputDF = Seq(
+      (null, LocalTime.parse("01:02:03"), LocalTime.parse("01:02:03")),
+      ("HOUR", null, LocalTime.parse("01:02:03")),
+      ("HOUR", LocalTime.parse("01:02:03"), null),
+      ("HOUR", null, null),
+      (null, LocalTime.parse("01:02:03"), null),
+      (null, null, LocalTime.parse("01:02:03")),
+      (null, null, null)
+    ).toDF("unit", "start", "end")
+    val nullResult = Seq[Integer](
+      null, null, null, null, null, null, null
+    ).toDF("diff").select(col("diff"))
+    checkAnswer(
+      nullInputDF.select(time_diff(col("unit"), col("start"), col("end"))),
+      nullResult
+    )
+
+    // Error is thrown for malformed input.
+    val invalidUnitDF = Seq(
+      ("invalid_unit", LocalTime.parse("01:02:03"), LocalTime.parse("01:02:03"))
+    ).toDF("unit", "start", "end")
+    checkError(
+      exception = intercept[SparkIllegalArgumentException] {
+        invalidUnitDF.select(time_diff(col("unit"), col("start"), col("end"))).collect()
+      },
+      condition = "INVALID_PARAMETER_VALUE.TIME_UNIT",
+      parameters = Map(
+        "functionName" -> "`time_diff`",
+        "parameter" -> "`unit`",
+        "invalidValue" -> "'invalid_unit'"
+      )
+    )
+  }
+
   test("SPARK-53107: time_trunc function") {
     // Input data for the function (including null values).
     val schema = StructType(Seq(