diff --git a/src/Microsoft.AspNetCore.NodeServices/HostingModels/OutOfProcessNodeInstance.cs b/src/Microsoft.AspNetCore.NodeServices/HostingModels/OutOfProcessNodeInstance.cs
index e85ed8c1..3c9cfd51 100644
--- a/src/Microsoft.AspNetCore.NodeServices/HostingModels/OutOfProcessNodeInstance.cs
+++ b/src/Microsoft.AspNetCore.NodeServices/HostingModels/OutOfProcessNodeInstance.cs
@@ -126,7 +126,7 @@ protected virtual ProcessStartInfo PrepareNodeProcessStartInfo(
             var existingNodePath = Environment.GetEnvironmentVariable("NODE_PATH") ?? string.Empty;
             if (existingNodePath != string.Empty)
             {
-                existingNodePath += ":";
+                existingNodePath += Path.PathSeparator;
             }
 
             var nodePathValue = existingNodePath + Path.Combine(projectPath, "node_modules");