diff --git a/aws_lambda_powertools/event_handler/api_gateway.py b/aws_lambda_powertools/event_handler/api_gateway.py
index 8e4ea866dd1..5d57c645b27 100644
--- a/aws_lambda_powertools/event_handler/api_gateway.py
+++ b/aws_lambda_powertools/event_handler/api_gateway.py
@@ -26,7 +26,7 @@
 _DYNAMIC_ROUTE_PATTERN = r"(<\w+>)"
 _SAFE_URI = "-._~()'!*:@,;"  # https://www.ietf.org/rfc/rfc3986.txt
 # API GW/ALB decode non-safe URI chars; we must support them too
-_UNSAFE_URI = "%<>\[\]{}|^"  # noqa: W605
+_UNSAFE_URI = "%<> \[\]{}|^"  # noqa: W605
 _NAMED_GROUP_BOUNDARY_PATTERN = fr"(?P\1[{_SAFE_URI}{_UNSAFE_URI}\\w]+)"
 
 
diff --git a/tests/functional/event_handler/test_api_gateway.py b/tests/functional/event_handler/test_api_gateway.py
index c21cd352961..9b5835c8b20 100644
--- a/tests/functional/event_handler/test_api_gateway.py
+++ b/tests/functional/event_handler/test_api_gateway.py
@@ -715,6 +715,42 @@ def get_network_account(account_id: str, network_id: str):
     app.resolve(event, {})
 
 
+def test_similar_dynamic_routes_with_whitespaces():
+    # GIVEN
+    app = ApiGatewayResolver()
+    event = deepcopy(LOAD_GW_EVENT)
+
+    # WHEN
+    # r'^/accounts/(?P<account_id>\\w+\\b)$' # noqa: E800
+    @app.get("/accounts/<account_id>")
+    def get_account(account_id: str):
+        assert account_id == "single account"
+
+    # r'^/accounts/(?P<account_id>\\w+\\b)/source_networks$' # noqa: E800
+    @app.get("/accounts/<account_id>/source_networks")
+    def get_account_networks(account_id: str):
+        assert account_id == "nested account"
+
+    # r'^/accounts/(?P<account_id>\\w+\\b)/source_networks/(?P<network_id>\\w+\\b)$' # noqa: E800
+    @app.get("/accounts/<account_id>/source_networks/<network_id>")
+    def get_network_account(account_id: str, network_id: str):
+        assert account_id == "nested account"
+        assert network_id == "network 123"
+
+    # THEN
+    event["resource"] = "/accounts/{account_id}"
+    event["path"] = "/accounts/single account"
+    assert app.resolve(event, {})["statusCode"] == 200
+
+    event["resource"] = "/accounts/{account_id}/source_networks"
+    event["path"] = "/accounts/nested account/source_networks"
+    assert app.resolve(event, {})["statusCode"] == 200
+
+    event["resource"] = "/accounts/{account_id}/source_networks/{network_id}"
+    event["path"] = "/accounts/nested account/source_networks/network 123"
+    assert app.resolve(event, {})["statusCode"] == 200
+
+
 @pytest.mark.parametrize(
     "req",
     [