diff --git a/src/ProjectTemplates/Shared/Project.cs b/src/ProjectTemplates/Shared/Project.cs
index b9e9e7d60a91..58df70e03eba 100644
--- a/src/ProjectTemplates/Shared/Project.cs
+++ b/src/ProjectTemplates/Shared/Project.cs
@@ -352,6 +352,16 @@ public async Task VerifyLaunchSettings(string[] expectedLaunchProfileNames)
         }
     }
 
+    public async Task VerifyHasProperty(string propertyName, string expectedValue)
+    {
+        var projectFile = Directory.EnumerateFiles(TemplateOutputDir, "*proj").FirstOrDefault();
+
+        Assert.NotNull(projectFile);
+
+        var projectFileContents = await File.ReadAllTextAsync(projectFile);
+        Assert.Contains($"<{propertyName}>{expectedValue}</{propertyName}>", projectFileContents);
+    }
+
     public string ReadFile(string path)
     {
         AssertFileExists(path, shouldExist: true);
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/Api-CSharp.csproj.in b/src/ProjectTemplates/Web.ProjectTemplates/Api-CSharp.csproj.in
index c42865b4b667..ae3d10f13686 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/Api-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/Api-CSharp.csproj.in
@@ -7,6 +7,7 @@
     <NoDefaultLaunchSettingsFile Condition="'$(ExcludeLaunchSettings)' == 'True'">true</NoDefaultLaunchSettingsFile>
     <RootNamespace Condition="'$(name)' != '$(name{-VALUE-FORMS-}safe_namespace)'">Company.WebApplication1</RootNamespace>
     <ServerGarbageCollection>false</ServerGarbageCollection>
+    <InvariantGlobalization>true</InvariantGlobalization>
     <!--#if (NativeAot) -->
     <PublishAot>true</PublishAot>
     <!--#endif -->
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/GrpcService-CSharp.csproj.in b/src/ProjectTemplates/Web.ProjectTemplates/GrpcService-CSharp.csproj.in
index 3554a4084796..3b003a21c4b3 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/GrpcService-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/GrpcService-CSharp.csproj.in
@@ -4,6 +4,7 @@
     <TargetFramework>${DefaultNetCoreTargetFramework}</TargetFramework>
     <Nullable>enable</Nullable>
     <ImplicitUsings>enable</ImplicitUsings>
+    <InvariantGlobalization>true</InvariantGlobalization>
     <!--#if (NativeAot) -->
     <PublishAot>true</PublishAot>
     <!--#endif -->
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/WebApi-CSharp.csproj.in b/src/ProjectTemplates/Web.ProjectTemplates/WebApi-CSharp.csproj.in
index 5b949b47fba0..e79188f36d01 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/WebApi-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/WebApi-CSharp.csproj.in
@@ -4,6 +4,7 @@
     <TargetFramework>${DefaultNetCoreTargetFramework}</TargetFramework>
     <Nullable>enable</Nullable>
     <ImplicitUsings>enable</ImplicitUsings>
+    <InvariantGlobalization>true</InvariantGlobalization>
     <UserSecretsId Condition="'$(IndividualAuth)' == 'True' OR '$(OrganizationalAuth)' == 'True'">aspnet-Company.WebApplication1-53bc9b9d-9d6a-45d4-8429-2a2761773502</UserSecretsId>
     <NoDefaultLaunchSettingsFile Condition="'$(ExcludeLaunchSettings)' == 'True'">True</NoDefaultLaunchSettingsFile>
     <RootNamespace Condition="'$(name)' != '$(name{-VALUE-FORMS-}safe_namespace)'">Company.WebApplication1</RootNamespace>
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/WebApi-FSharp.fsproj.in b/src/ProjectTemplates/Web.ProjectTemplates/WebApi-FSharp.fsproj.in
index 8a9f8ed0e2e5..2065714d5cb2 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/WebApi-FSharp.fsproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/WebApi-FSharp.fsproj.in
@@ -2,6 +2,7 @@
 
   <PropertyGroup>
     <TargetFramework>${DefaultNetCoreTargetFramework}</TargetFramework>
+    <InvariantGlobalization>true</InvariantGlobalization>
     <NoDefaultLaunchSettingsFile Condition="'$(ExcludeLaunchSettings)' == 'True'">True</NoDefaultLaunchSettingsFile>
     <RootNamespace Condition="'$(name)' != '$(name{-VALUE-FORMS-}safe_namespace)'">Company.WebApplication1</RootNamespace>
   </PropertyGroup>
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/Worker-CSharp.csproj.in b/src/ProjectTemplates/Web.ProjectTemplates/Worker-CSharp.csproj.in
index 19004ba1c8e9..85186fbef735 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/Worker-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/Worker-CSharp.csproj.in
@@ -4,6 +4,7 @@
     <TargetFramework>${DefaultNetCoreTargetFramework}</TargetFramework>
     <Nullable>enable</Nullable>
     <ImplicitUsings>enable</ImplicitUsings>
+    <InvariantGlobalization>true</InvariantGlobalization>
     <UserSecretsId>dotnet-Company.Application1-53bc9b9d-9d6a-45d4-8429-2a2761773502</UserSecretsId>
     <NoDefaultLaunchSettingsFile Condition="'$(ExcludeLaunchSettings)' == 'True'">True</NoDefaultLaunchSettingsFile>
     <RootNamespace Condition="'$(name)' != '$(name{-VALUE-FORMS-}safe_namespace)'">Company.Application1</RootNamespace>
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/Worker-FSharp.fsproj.in b/src/ProjectTemplates/Web.ProjectTemplates/Worker-FSharp.fsproj.in
index c3905c0a7424..c106066e6eff 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/Worker-FSharp.fsproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/Worker-FSharp.fsproj.in
@@ -3,6 +3,7 @@
   <PropertyGroup>
     <TargetFramework>${DefaultNetCoreTargetFramework}</TargetFramework>
     <UserSecretsId>dotnet-Company.Application1-53bc9b9d-9d6a-45d4-8429-2a2761773502</UserSecretsId>
+    <InvariantGlobalization>true</InvariantGlobalization>
     <NoDefaultLaunchSettingsFile Condition="'$(ExcludeLaunchSettings)' == 'True'">True</NoDefaultLaunchSettingsFile>
     <RootNamespace Condition="'$(name)' != '$(name{-VALUE-FORMS-}safe_namespace)'">Company.Application1</RootNamespace>
   </PropertyGroup>
diff --git a/src/ProjectTemplates/test/Templates.Tests/ApiTemplateTest.cs b/src/ProjectTemplates/test/Templates.Tests/ApiTemplateTest.cs
index 319c7054207e..62bf4a7f3ad3 100644
--- a/src/ProjectTemplates/test/Templates.Tests/ApiTemplateTest.cs
+++ b/src/ProjectTemplates/test/Templates.Tests/ApiTemplateTest.cs
@@ -65,6 +65,8 @@ private async Task ApiTemplateCore(string languageOverride, string[] args = null
             : new[] { "http", "IIS Express" };
         await project.VerifyLaunchSettings(expectedLaunchProfileNames);
 
+        await project.VerifyHasProperty("InvariantGlobalization", "true");
+
         // Avoid the F# compiler. See https://github.com/dotnet/aspnetcore/issues/14022
         if (languageOverride != null)
         {
diff --git a/src/ProjectTemplates/test/Templates.Tests/GrpcTemplateTest.cs b/src/ProjectTemplates/test/Templates.Tests/GrpcTemplateTest.cs
index 7c4385d56e8c..e0d4fdecce8e 100644
--- a/src/ProjectTemplates/test/Templates.Tests/GrpcTemplateTest.cs
+++ b/src/ProjectTemplates/test/Templates.Tests/GrpcTemplateTest.cs
@@ -75,6 +75,8 @@ private async Task GrpcTemplateCore(string[] args = null)
         var expectedLaunchProfileNames = new[] { "http", "https" };
         await project.VerifyLaunchSettings(expectedLaunchProfileNames);
 
+        await project.VerifyHasProperty("InvariantGlobalization", "true");
+
         await project.RunDotNetPublishAsync();
 
         await project.RunDotNetBuildAsync();
diff --git a/src/ProjectTemplates/test/Templates.Tests/WorkerTemplateTest.cs b/src/ProjectTemplates/test/Templates.Tests/WorkerTemplateTest.cs
index 4c1ad90756e4..46ca63e0c569 100644
--- a/src/ProjectTemplates/test/Templates.Tests/WorkerTemplateTest.cs
+++ b/src/ProjectTemplates/test/Templates.Tests/WorkerTemplateTest.cs
@@ -40,6 +40,8 @@ public async Task WorkerTemplateAsync(string language, string[] args)
 
         await project.RunDotNetNewAsync("worker", language: language, args: args);
 
+        await project.VerifyHasProperty("InvariantGlobalization", "true");
+
         await project.RunDotNetPublishAsync();
 
         // Run dotnet build after publish. The reason is that one uses Config = Debug and the other uses Config = Release