diff --git a/core/src/components/action-sheet/action-sheet.tsx b/core/src/components/action-sheet/action-sheet.tsx
index bf03534de42..5c1dcc26617 100644
--- a/core/src/components/action-sheet/action-sheet.tsx
+++ b/core/src/components/action-sheet/action-sheet.tsx
@@ -239,16 +239,18 @@ export class ActionSheet implements ComponentInterface, OverlayInterface {
   }
 
   render() {
-    const { htmlAttributes } = this;
+    const { header, htmlAttributes, overlayIndex } = this;
     const mode = getIonMode(this);
     const allButtons = this.getButtons();
     const cancelButton = allButtons.find((b) => b.role === 'cancel');
     const buttons = allButtons.filter((b) => b.role !== 'cancel');
+    const headerID = `action-sheet-${overlayIndex}-header`;
 
     return (
       <Host
         role="dialog"
         aria-modal="true"
+        aria-labelledby={header !== undefined ? headerID : null}
         tabindex="-1"
         {...(htmlAttributes as any)}
         style={{
@@ -256,7 +258,6 @@ export class ActionSheet implements ComponentInterface, OverlayInterface {
         }}
         class={{
           [mode]: true,
-
           ...getClassMap(this.cssClass),
           'overlay-hidden': true,
           'action-sheet-translucent': this.translucent,
@@ -268,17 +269,18 @@ export class ActionSheet implements ComponentInterface, OverlayInterface {
 
         <div tabindex="0"></div>
 
-        <div class="action-sheet-wrapper ion-overlay-wrapper" role="dialog" ref={(el) => (this.wrapperEl = el)}>
+        <div class="action-sheet-wrapper ion-overlay-wrapper" ref={(el) => (this.wrapperEl = el)}>
           <div class="action-sheet-container">
             <div class="action-sheet-group" ref={(el) => (this.groupEl = el)}>
-              {this.header !== undefined && (
+              {header !== undefined && (
                 <div
+                  id={headerID}
                   class={{
                     'action-sheet-title': true,
                     'action-sheet-has-sub-title': this.subHeader !== undefined,
                   }}
                 >
-                  {this.header}
+                  {header}
                   {this.subHeader && <div class="action-sheet-sub-title">{this.subHeader}</div>}
                 </div>
               )}
diff --git a/core/src/components/action-sheet/test/a11y/action-sheet.e2e.ts b/core/src/components/action-sheet/test/a11y/action-sheet.e2e.ts
new file mode 100644
index 00000000000..7412b2ae885
--- /dev/null
+++ b/core/src/components/action-sheet/test/a11y/action-sheet.e2e.ts
@@ -0,0 +1,57 @@
+import AxeBuilder from '@axe-core/playwright';
+import { expect } from '@playwright/test';
+import type { E2EPage } from '@utils/test/playwright';
+import { test } from '@utils/test/playwright';
+
+const testAria = async (page: E2EPage, buttonID: string, expectedAriaLabelledBy: string | null) => {
+  const didPresent = await page.spyOnEvent('ionActionSheetDidPresent');
+  const button = page.locator(`#${buttonID}`);
+
+  await button.click();
+  await didPresent.next();
+
+  const alert = page.locator('ion-action-sheet');
+
+  /**
+   * expect().toHaveAttribute() can't check for a null value, so grab and check
+   * the value manually instead.
+   */
+  const ariaLabelledBy = await alert.getAttribute('aria-labelledby');
+
+  expect(ariaLabelledBy).toBe(expectedAriaLabelledBy);
+};
+
+test.describe('action-sheet: a11y', () => {
+  test.beforeEach(async ({ page, skip }) => {
+    skip.rtl();
+    await page.goto(`/src/components/action-sheet/test/a11y`);
+  });
+
+  test('should not have accessibility violations when header is defined', async ({ page }) => {
+    const button = page.locator('#bothHeaders');
+    const didPresent = await page.spyOnEvent('ionActionSheetDidPresent');
+
+    await button.click();
+    await didPresent.next();
+
+    /**
+     * action-sheet overlays the entire screen, so
+     * Axe will be unable to verify color contrast
+     * on elements under the backdrop.
+     */
+    const results = await new AxeBuilder({ page }).disableRules('color-contrast').analyze();
+    expect(results.violations).toEqual([]);
+  });
+
+  test('should have aria-labelledby when header is set', async ({ page }) => {
+    await testAria(page, 'bothHeaders', 'action-sheet-1-header');
+  });
+
+  test('should not have aria-labelledby when header is not set', async ({ page }) => {
+    await testAria(page, 'noHeaders', null);
+  });
+
+  test('should allow for manually specifying aria attributes', async ({ page }) => {
+    await testAria(page, 'customAria', 'Custom title');
+  });
+});
diff --git a/core/src/components/action-sheet/test/a11y/index.html b/core/src/components/action-sheet/test/a11y/index.html
new file mode 100644
index 00000000000..566b7dcdc1b
--- /dev/null
+++ b/core/src/components/action-sheet/test/a11y/index.html
@@ -0,0 +1,68 @@
+<!DOCTYPE html>
+<html lang="en" dir="ltr">
+  <head>
+    <meta charset="UTF-8" />
+    <title>Action Sheet - A11y</title>
+    <meta name="viewport" content="width=device-width, initial-scale=1.0, minimum-scale=1.0" />
+    <link href="../../../../../css/ionic.bundle.css" rel="stylesheet" />
+    <link href="../../../../../scripts/testing/styles.css" rel="stylesheet" />
+    <script src="../../../../../scripts/testing/scripts.js"></script>
+    <script nomodule src="../../../../../dist/ionic/ionic.js"></script>
+    <script type="module" src="../../../../../dist/ionic/ionic.esm.js"></script>
+  </head>
+  <script type="module">
+    import { actionSheetController } from '../../../../dist/ionic/index.esm.js';
+    window.actionSheetController = actionSheetController;
+  </script>
+
+  <body>
+    <main class="ion-padding">
+      <h1>Action Sheet - A11y</h1>
+
+      <ion-button id="bothHeaders" expand="block" onclick="presentBothHeaders()">Both Headers</ion-button>
+      <ion-button id="subHeaderOnly" expand="block" onclick="presentSubHeaderOnly()">Subheader Only</ion-button>
+      <ion-button id="noHeaders" expand="block" onclick="presentNoHeaders()">No Headers</ion-button>
+      <ion-button id="customAria" expand="block" onclick="presentCustomAria()">Custom Aria</ion-button>
+    </main>
+
+    <script>
+      async function openActionSheet(opts) {
+        const actionSheet = await actionSheetController.create(opts);
+        await actionSheet.present();
+      }
+
+      function presentBothHeaders() {
+        openActionSheet({
+          header: 'Header',
+          subHeader: 'Subtitle',
+          buttons: ['Confirm'],
+        });
+      }
+
+      function presentSubHeaderOnly() {
+        openActionSheet({
+          subHeader: 'Subtitle',
+          buttons: ['Confirm'],
+        });
+      }
+
+      function presentNoHeaders() {
+        openActionSheet({
+          buttons: ['Confirm'],
+        });
+      }
+
+      function presentCustomAria() {
+        openActionSheet({
+          header: 'Header',
+          subHeader: 'Subtitle',
+          buttons: ['Confirm'],
+          htmlAttributes: {
+            'aria-labelledby': 'Custom title',
+            'aria-describedby': 'Custom description',
+          },
+        });
+      }
+    </script>
+  </body>
+</html>