From 2f54dfc0d176c14c7d279829743533497090a575 Mon Sep 17 00:00:00 2001 From: Antonio Gonzalez Date: Wed, 16 Aug 2017 07:12:00 -0600 Subject: [PATCH 1/6] fix #2211 --- qiita_db/metadata_template/test/test_util.py | 14 +++++++------- qiita_db/metadata_template/util.py | 5 ++++- 2 files changed, 11 insertions(+), 8 deletions(-) diff --git a/qiita_db/metadata_template/test/test_util.py b/qiita_db/metadata_template/test/test_util.py index 40d7cc6cc..51d6b3a39 100644 --- a/qiita_db/metadata_template/test/test_util.py +++ b/qiita_db/metadata_template/test/test_util.py @@ -386,21 +386,21 @@ def test_get_pgsql_reserved_words(self): "Value for sample 3\n") EXP_SAMPLE_TEMPLATE_SPACES_EMPTY_ROW = ( - "sample_name\tcollection_timestamp\tdescription\thas_extracted_data\t" - "has_physical_specimen\thost_subject_id\tint_column\tlatitude\tlongitude\t" - "physical_location\trequired_sample_info_status\tsample_type\t" - "str_column\n" - "2.Sample1 \t2014-05-29 12:24:51\tTest Sample 1\tTrue\tTrue\t" + "\x00sample_name\tcollection_timestamp\tdescription\thas_extracted_data\t" + "\x01has_physical_specimen\thost_subject_id\tint_column\tlatitude\t" + "longitude\t physical_location\trequired_sample_info_status" + "\tsample_type\tstr_column\n" + " 2.Sample1 \t2014-05-29 12:24:51\tTest Sample 1\tTrue\tTrue\t" "NotIdentified\t1\t42.42\t41.41\tlocation1\treceived\ttype1\t" "Value for sample 1\n" - "2.Sample2 \t2014-05-29 12:24:51\t" + " 2.Sample2 \t2014-05-29 12:24:51\t" "Test Sample 2\tTrue\tTrue\tNotIdentified\t2\t4.2\t1.1\tlocation1\t" "received\ttype1\tValue for sample 2\n" "2.Sample3\t2014-05-29 12:24:51\tTest Sample 3\tTrue\t" "True\tNotIdentified\t3\t4.8\t4.41\tlocation1\treceived\ttype1\t" "Value for sample 3\n" "\t\t\t\t\t\t\t\t\t\t\t\t\n" - "\t\t\t\t\t\t\t\t\t\t\t\t\n") + "\t\t\t\t\t\t\t\t\t\t \t\t\n") EXP_ST_SPACES_EMPTY_COLUMN = ( "sample_name\tcollection_timestamp\tdescription\thas_extracted_data\t" diff --git a/qiita_db/metadata_template/util.py b/qiita_db/metadata_template/util.py index ce2b520da..b43b647c6 100644 --- a/qiita_db/metadata_template/util.py +++ b/qiita_db/metadata_template/util.py @@ -10,6 +10,7 @@ from collections import defaultdict from future.utils import PY3, viewitems from six import StringIO +from string import printable import pandas as pd import numpy as np @@ -103,7 +104,9 @@ def load_template_to_dataframe(fn, index='sample_name'): # Load in file lines holdfile = None with open_file(fn, mode='U') as f: - holdfile = f.readlines() + # here we are removing all non printable chars AKA non UTF-8 chars + holdfile = [''.join([c for c in l if c in printable]) + for l in f.readlines()] if not holdfile: raise ValueError('Empty file passed!') From b2c934efc54ee5ace3ed587bc488afbd7cde2dc0 Mon Sep 17 00:00:00 2001 From: Antonio Gonzalez Date: Wed, 16 Aug 2017 12:07:13 -0600 Subject: [PATCH 2/6] following @qiita-dev suggestions --- qiita_db/metadata_template/test/test_util.py | 6 +++--- qiita_db/metadata_template/util.py | 18 ++++++++++++++---- 2 files changed, 17 insertions(+), 7 deletions(-) diff --git a/qiita_db/metadata_template/test/test_util.py b/qiita_db/metadata_template/test/test_util.py index 51d6b3a39..db7557d8f 100644 --- a/qiita_db/metadata_template/test/test_util.py +++ b/qiita_db/metadata_template/test/test_util.py @@ -149,7 +149,7 @@ def test_load_template_to_dataframe_lowercase(self): def test_load_template_to_dataframe_non_utf8(self): bad = EXP_SAMPLE_TEMPLATE.replace('Test Sample 2', 'Test Sample\x962') - with self.assertRaises(qdb.exceptions.QiitaDBError): + with self.assertRaises(ValueError): qdb.metadata_template.util.load_template_to_dataframe( StringIO(bad)) @@ -386,8 +386,8 @@ def test_get_pgsql_reserved_words(self): "Value for sample 3\n") EXP_SAMPLE_TEMPLATE_SPACES_EMPTY_ROW = ( - "\x00sample_name\tcollection_timestamp\tdescription\thas_extracted_data\t" - "\x01has_physical_specimen\thost_subject_id\tint_column\tlatitude\t" + "sample_name\tcollection_timestamp\tdescription\thas_extracted_data\t" + "has_physical_specimen\thost_subject_id\tint_column\tlatitude\t" "longitude\t physical_location\trequired_sample_info_status" "\tsample_type\tstr_column\n" " 2.Sample1 \t2014-05-29 12:24:51\tTest Sample 1\tTrue\tTrue\t" diff --git a/qiita_db/metadata_template/util.py b/qiita_db/metadata_template/util.py index b43b647c6..59a649053 100644 --- a/qiita_db/metadata_template/util.py +++ b/qiita_db/metadata_template/util.py @@ -104,9 +104,19 @@ def load_template_to_dataframe(fn, index='sample_name'): # Load in file lines holdfile = None with open_file(fn, mode='U') as f: - # here we are removing all non printable chars AKA non UTF-8 chars - holdfile = [''.join([c for c in l if c in printable]) - for l in f.readlines()] + errors = [] + holdfile = f.readlines() + # here we are checking for non printable chars AKA non UTF-8 chars + for row, line in enumerate(holdfile): + for col, block in enumerate(line.split('\t')): + tblock = ''.join([c for c in block if c in printable]) + if len(block) != len(tblock): + errors.append('(%d, %d)' % (row, col)) + if errors: + raise ValueError( + "There are non valid UTF-8 characters in the following " + "positions (row, col): %s" % ', '.join(errors)) + if not holdfile: raise ValueError('Empty file passed!') @@ -140,7 +150,7 @@ def load_template_to_dataframe(fn, index='sample_name'): # .strip will remove odd chars, newlines, tabs and multiple # spaces but we need to read a new line at the end of the # line(+'\n') - newcols = [d.strip(" \r\x0b\x0c\n") for d in cols] + newcols = [d.strip(" \r\n") for d in cols] holdfile[pos] = '\t'.join(newcols) + '\n' From 5396a3470191fcccd88651d6331038efe545213b Mon Sep 17 00:00:00 2001 From: Antonio Gonzalez Date: Thu, 17 Aug 2017 09:07:11 -0600 Subject: [PATCH 3/6] fixing errors and improving message --- qiita_db/metadata_template/util.py | 58 +- qiita_ware/test/test_dispatchable.py | 5 +- .../studies/1235/prep_template_1235.txt | 536 +-- .../studies/1288/prep_template_1288.txt | 3012 ++++++++--------- 4 files changed, 1801 insertions(+), 1810 deletions(-) diff --git a/qiita_db/metadata_template/util.py b/qiita_db/metadata_template/util.py index 59a649053..3807a1f0a 100644 --- a/qiita_db/metadata_template/util.py +++ b/qiita_db/metadata_template/util.py @@ -7,7 +7,6 @@ # ----------------------------------------------------------------------------- from __future__ import division -from collections import defaultdict from future.utils import PY3, viewitems from six import StringIO from string import printable @@ -104,18 +103,24 @@ def load_template_to_dataframe(fn, index='sample_name'): # Load in file lines holdfile = None with open_file(fn, mode='U') as f: - errors = [] + errors = {} holdfile = f.readlines() # here we are checking for non printable chars AKA non UTF-8 chars for row, line in enumerate(holdfile): for col, block in enumerate(line.split('\t')): tblock = ''.join([c for c in block if c in printable]) if len(block) != len(tblock): - errors.append('(%d, %d)' % (row, col)) - if errors: + tblock = ''.join([c if c in printable else '♥' + for c in block]) + if tblock not in errors: + errors[tblock] = [] + errors[tblock].append('(%d, %d)' % (row, col)) + if bool(errors): raise ValueError( - "There are non valid UTF-8 characters in the following " - "positions (row, col): %s" % ', '.join(errors)) + "There are non valid UTF-8 characters. The errors are " + "shown as ♥: %s" % '; '.join( + ['%s = %s' % (k, ', '.join(v)) + for k, v in viewitems(errors)])) if not holdfile: raise ValueError('Empty file passed!') @@ -162,34 +167,19 @@ def load_template_to_dataframe(fn, index='sample_name'): # comment: # using the tab character as "comment" we remove rows that are # constituted only by delimiters i. e. empty rows. - try: - template = pd.read_csv( - StringIO(''.join(holdfile)), - sep='\t', - dtype=str, - encoding='utf-8', - infer_datetime_format=False, - keep_default_na=False, - index_col=False, - comment='\t', - converters={index: lambda x: str(x).strip()}) - # remove newlines and tabs from fields - template.replace(to_replace='[\t\n\r\x0b\x0c]+', value='', - regex=True, inplace=True) - except UnicodeDecodeError: - # Find row number and col number for utf-8 encoding errors - headers = holdfile[0].strip().split('\t') - errors = defaultdict(list) - for row, line in enumerate(holdfile, 1): - for col, cell in enumerate(line.split('\t')): - try: - cell.encode('utf-8') - except UnicodeError: - errors[headers[col]].append(row) - lines = ['%s: row(s) %s' % (header, ', '.join(map(str, rows))) - for header, rows in viewitems(errors)] - raise qdb.exceptions.QiitaDBError( - 'Non UTF-8 characters found in columns:\n' + '\n'.join(lines)) + template = pd.read_csv( + StringIO(''.join(holdfile)), + sep='\t', + dtype=str, + encoding='utf-8', + infer_datetime_format=False, + keep_default_na=False, + index_col=False, + comment='\t', + converters={index: lambda x: str(x).strip()}) + # remove newlines and tabs from fields + template.replace(to_replace='[\t\n\r\x0b\x0c]+', value='', + regex=True, inplace=True) initial_columns = set(template.columns) diff --git a/qiita_ware/test/test_dispatchable.py b/qiita_ware/test/test_dispatchable.py index 7a3eeb377..711838efc 100644 --- a/qiita_ware/test/test_dispatchable.py +++ b/qiita_ware/test/test_dispatchable.py @@ -84,8 +84,9 @@ def test_create_sample_template_nonutf8(self): 'sample_info_utf8_error.txt') obs = create_sample_template(fp, Study(1), False) exp = {'status': 'danger', - 'message': u"Non UTF-8 characters found in columns:" - u"\n\ufffdcollection_timestamp: row(s) 1"} + 'message': "There are non valid UTF-8 characters. The errors " + "are shown as ♥: ♥collection_timestamp " + "= (0, 13)"} self.assertEqual(obs, exp) def test_update_sample_template(self): diff --git a/test_data_studies/studies/1235/prep_template_1235.txt b/test_data_studies/studies/1235/prep_template_1235.txt index 6c9f163b9..78d5e459f 100644 --- a/test_data_studies/studies/1235/prep_template_1235.txt +++ b/test_data_studies/studies/1235/prep_template_1235.txt @@ -1,269 +1,269 @@ sample_name barcodesequence center_name experiment_design_description center_project_name KEY_SEQ library_construction_protocol LINKER platform illumina_technology linkerprimersequence REGION RUN_CENTER RUN_DATE run_prefix SAMPLE_CENTER STUDY_CENTER samp_size sequencing_meth target_gene target_subfragment pcr_primers emp_status -sp0192 GATCAACCCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0193 CGTCCGTATGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0195 CTAAGACGTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0200 GAAGAGGGTTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0310 ACACAGTCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0314 GCTTCCAGACAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0317 TTCACCTGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0325 ATTATACGGCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0329 GCGCCGAATCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0333 ATAAAGAGGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0339 ATTCAGATGGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0340 GACTCAACCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0341 CGTATAAATGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0342 CAACTAGACTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0343 AACCTCGGATAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0344 TGCCGCCGTAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0345 GCCTCGTACTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0346 CACGAGCTACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0347 CGCATACGACCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0349 GTGCTTGTGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0350 ACCAACAGATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0351 CATTTGACGACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0352 GTGGCCTACTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0353 TCTCGATAAGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0354 TTCCCTTCTCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0355 AAGTGAAGCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0368 GTAGTGTCAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0370 CATCTGGGCAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0371 AACCCAGATGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0372 AATACAGACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0373 GTCGGAAATTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0374 TCTAACGAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0375 ACTCGGCCAACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0376 AGTGCCCTTGGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0379 TGTCCGTGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0380 GTTGGTTGGCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0381 GGAAGAAGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0382 ACGTAACCACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0383 CACCTGTAGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0384 TTCCACACGTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0701 GGCTCAGATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0702 AGTCCGAGTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0703 GACTCTGCTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0705 TGGTTCATCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0706 ATGAATGCGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0714 CAGAGCTAATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0715 ACTAGTTGGACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0721 AGTAGGAGGCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0722 ATGCGAGACTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0724 AGGTCATCTTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0727 ACACTTCGGCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0729 GTGCTGCGCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0731 TGCTGTGACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0733 GTCCAGCTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0734 TAGTAGCACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0736 CACGTACACGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0738 AGTAGACTTACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0739 CCGCGATTTCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0740 ACCTCCCGGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0742 AGCACTTTGAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0743 CGATTAGGAATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0747 ATTCCTCTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0752 GCTATTCCTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0753 CTCAAGTCAAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0754 CACTGAGTACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0755 TCGTTTCTTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0756 GGATGCAGGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0757 TTGCAAGTACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0758 GCACTTCATTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0759 TTGCCCTTTGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0767 TTGTTACGTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0768 AATGTCACCAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0769 GTTCTGCTTGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1000 ACGTCTCAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1001 ACACCGCACAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1004 ACTCATCTTCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1005 ACCCGGATTTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1006 TTATCCAGTCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1007 CGCTTGTGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1010 TACGCCCATCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1011 GTAGCACTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1012 AATCTTGCGCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1013 GCATGCATCCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1014 TAGACACCGTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1015 GAAACTCCTAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1016 GACCGTCAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1017 GCCTGCAGTACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1018 AGACAAGCTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1019 CTTGGTAGTGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1020 TCCTAGGTCCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1021 TCCAGGGCTATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1023 TTATGTACGGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1026 ATCGGGCTTAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1027 GAGATACAGTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1028 TCCGCAACCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1029 TCCTCACTATCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1030 AAGATCGTACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1031 TGGAGAGGAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1032 TTGGACGTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1033 ATCGATCCACAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1034 TTGGAACGGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1035 GATCTAATCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1036 GCGTAGAGAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1037 TCACTTGGTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1038 GTCTCCTCCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1039 GGAAATCCCATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1083 CTCCAGGTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1087 TAAACGCGACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1089 ATCCCAGCATGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1098 CCTCGGGTACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1100 CAGGATTCGTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1140 CAAACTGCGTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1142 CTTTAGCGCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1144 GACCGATAGGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1145 CAAGTCGAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1147 CGGCACTATCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1149 CTCGGTCAACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1151 ACACACCCTGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1155 TTAAGCGCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1157 AAGCGTACATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1158 TCGCGCAACTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1160 TCCGTTCGTTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1163 ATGTTTAGACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1164 GGCACACCCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1165 ATTCCCAGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1261 GCAGCCATATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1268 GAATGACGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1271 CATCGGATCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1272 GTCAGCCGTTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1273 ACCTAGCTAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1274 ACGCCTTTCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1275 GTCCACTTGGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1276 CACAAAGCGATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1277 GTTACAGTTGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1278 GGACTCAACTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1279 GAAGATCTATCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1280 CCTTTCACCTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1281 TCTCGCACTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1282 GGACCGCTTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1284 GAATCCTCACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1286 CGCGTCAAACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1287 CATGTTGGAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1288 GTCAAGACCTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1294 CAGCCTGCAAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1295 GTCTCTGAAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1298 CCACTTGAGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1299 ATGGGACCTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1300 AGAATCCACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1304 ATAAGGTCGCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1307 AGTACCTAAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1308 CGAGATAGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1309 GCTTCTCTCACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1310 GTACCTAGCCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1312 CAGTTCGAGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1314 TCAAGCAATACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1315 CCAGTATCGCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1338 TGCGGGATTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1341 ACCACCGTAACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1342 AGAATAGCGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1344 ACATGTCACGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1345 GGCGAACTGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1347 GCAAGTGTGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1348 CCTGGAATTAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1374 CGGACTCGTTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1376 TTCTGGTCTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1377 CACGGTCCTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1378 GTCCTGACACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1379 CACCGTGACACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1380 GATTTAGAGGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1384 ACGGTTTCTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1386 TCACGAGTCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1389 ATAGGTGTGCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1390 CATGTCTTCCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1392 ATACGGGTTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1395 ACTTACGCCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1399 TTGGTGCCTGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1400 GACGGAACAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1402 TTAGACTCGGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1403 CGTACCAGATCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1404 AGGTGGTGGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1405 AGACGTTGCTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1407 CTGGTCTTACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1409 ACCCTATTGCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1410 TGGAAACCATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1520 ATCAGCCAGCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1525 TTACACAAAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1526 TTCTCGGTTCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1527 ACACATAAGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1529 ACGACGCATTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1532 AAGGAGTGCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1533 TCGTAAGCCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1535 TGACGCCTCCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1536 CCAAACTCGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1540 AGCCTCATGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1541 TGAATCGAAGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1542 TTGACACACGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1543 AAGTATCCTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1545 TTACGTGGCGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1546 TGTACGGATAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1547 CTTAGCTACTCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1548 GGAACGACGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1550 GTGACGTTAGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1552 CTGGTGCTGAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1553 CACGTTTATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1554 GTCTGTTGAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1555 TCAGACCAACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1557 TCGTCGCCAAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1560 GGTCGTGTCTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1561 GTGAGGGCAAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1565 CCACGGTACTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1566 CAAGCGTTGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1568 CGTGGGCTCATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1573 GAGTCTTGGTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1576 GCCCAAGTTCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1579 GACTTATGCCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1580 TGAGTTCGGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1582 GACAGAGGTGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1584 GAAACGGAAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1585 AGTGATGTGACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1587 AACATGCATGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1588 CGTCGTCTAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1590 TCGGTCCATAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1591 AATCAACTAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1597 CAATGCCTCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1598 TGTCAGCTGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1599 CATTTCGCACTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1601 CCTTCTGTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1608 TCAATGACCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1609 GTCGCTTGCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1610 CCAATGATAAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1611 ATCGAATCGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1612 CGGATTGCTGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1613 GTTAATGGCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1614 CTCGTTTCAGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1615 AATATCGGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1616 CTCTCATATGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1617 CTTGCATACCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1620 GTATGGAGCTAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1626 CTAGCAGTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1627 ACGCTGTCGGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1631 TCTACCACGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1632 CTATCGGAAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1635 CTTCCGCAGACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1636 GGTACTGTACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1637 TCTGGGCATTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1639 GTGCACGATAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1640 TAGTGCATTCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1641 TTAAACCGCGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1642 TCAGGACGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1644 GCGAACCTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1645 GAATATACCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1647 GAAAGGTGAGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1648 GGTCTAGGTCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1649 GCACTATACGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1926 GCTACTGGTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1927 GCTCCACAACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1928 GAGTTTACGGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1929 ACGTGAGGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1931 GTATAGTCCGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1932 TACTGCCAGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1933 TATAGGCTCCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1934 CGTCACTCCAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1935 CAAATGGTCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1936 TGAGACCCTACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1938 CTGCAGTAAGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1941 GTGTATCGCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1942 AGGGAAAGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1944 GTTATGACGGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 ÊC for 45 s, 50 ÊC for 60 s, and 72 ÊC for 90 s; a _nal extension of 10 min at 72 ÊC was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0192 GATCAACCCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0193 CGTCCGTATGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0195 CTAAGACGTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0200 GAAGAGGGTTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0310 ACACAGTCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0314 GCTTCCAGACAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0317 TTCACCTGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0325 ATTATACGGCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0329 GCGCCGAATCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0333 ATAAAGAGGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0339 ATTCAGATGGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0340 GACTCAACCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0341 CGTATAAATGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0342 CAACTAGACTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0343 AACCTCGGATAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0344 TGCCGCCGTAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0345 GCCTCGTACTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0346 CACGAGCTACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0347 CGCATACGACCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0349 GTGCTTGTGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0350 ACCAACAGATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0351 CATTTGACGACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0352 GTGGCCTACTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0353 TCTCGATAAGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0354 TTCCCTTCTCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0355 AAGTGAAGCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0368 GTAGTGTCAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0370 CATCTGGGCAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0371 AACCCAGATGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0372 AATACAGACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0373 GTCGGAAATTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0374 TCTAACGAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0375 ACTCGGCCAACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0376 AGTGCCCTTGGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0379 TGTCCGTGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0380 GTTGGTTGGCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0381 GGAAGAAGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0382 ACGTAACCACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0383 CACCTGTAGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0384 TTCCACACGTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0701 GGCTCAGATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0702 AGTCCGAGTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0703 GACTCTGCTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0705 TGGTTCATCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0706 ATGAATGCGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0714 CAGAGCTAATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0715 ACTAGTTGGACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0721 AGTAGGAGGCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0722 ATGCGAGACTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0724 AGGTCATCTTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0727 ACACTTCGGCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0729 GTGCTGCGCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0731 TGCTGTGACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0733 GTCCAGCTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0734 TAGTAGCACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0736 CACGTACACGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0738 AGTAGACTTACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0739 CCGCGATTTCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0740 ACCTCCCGGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0742 AGCACTTTGAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0743 CGATTAGGAATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0747 ATTCCTCTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0752 GCTATTCCTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0753 CTCAAGTCAAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0754 CACTGAGTACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0755 TCGTTTCTTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0756 GGATGCAGGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0757 TTGCAAGTACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0758 GCACTTCATTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0759 TTGCCCTTTGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0767 TTGTTACGTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0768 AATGTCACCAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0769 GTTCTGCTTGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1000 ACGTCTCAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1001 ACACCGCACAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1004 ACTCATCTTCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1005 ACCCGGATTTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1006 TTATCCAGTCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1007 CGCTTGTGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1010 TACGCCCATCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1011 GTAGCACTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1012 AATCTTGCGCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1013 GCATGCATCCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1014 TAGACACCGTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1015 GAAACTCCTAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1016 GACCGTCAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1017 GCCTGCAGTACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1018 AGACAAGCTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1019 CTTGGTAGTGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1020 TCCTAGGTCCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1021 TCCAGGGCTATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1023 TTATGTACGGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1026 ATCGGGCTTAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1027 GAGATACAGTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1028 TCCGCAACCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1029 TCCTCACTATCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1030 AAGATCGTACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1031 TGGAGAGGAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1032 TTGGACGTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1033 ATCGATCCACAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1034 TTGGAACGGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1035 GATCTAATCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1036 GCGTAGAGAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1037 TCACTTGGTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1038 GTCTCCTCCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1039 GGAAATCCCATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1083 CTCCAGGTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1087 TAAACGCGACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1089 ATCCCAGCATGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1098 CCTCGGGTACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1100 CAGGATTCGTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1140 CAAACTGCGTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1142 CTTTAGCGCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1144 GACCGATAGGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1145 CAAGTCGAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1147 CGGCACTATCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1149 CTCGGTCAACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1151 ACACACCCTGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1155 TTAAGCGCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1157 AAGCGTACATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1158 TCGCGCAACTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1160 TCCGTTCGTTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1163 ATGTTTAGACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1164 GGCACACCCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1165 ATTCCCAGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1261 GCAGCCATATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1268 GAATGACGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1271 CATCGGATCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1272 GTCAGCCGTTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1273 ACCTAGCTAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1274 ACGCCTTTCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1275 GTCCACTTGGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1276 CACAAAGCGATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1277 GTTACAGTTGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1278 GGACTCAACTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1279 GAAGATCTATCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1280 CCTTTCACCTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1281 TCTCGCACTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1282 GGACCGCTTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1284 GAATCCTCACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1286 CGCGTCAAACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1287 CATGTTGGAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1288 GTCAAGACCTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1294 CAGCCTGCAAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1295 GTCTCTGAAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1298 CCACTTGAGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1299 ATGGGACCTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1300 AGAATCCACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1304 ATAAGGTCGCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1307 AGTACCTAAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1308 CGAGATAGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1309 GCTTCTCTCACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1310 GTACCTAGCCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1312 CAGTTCGAGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1314 TCAAGCAATACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1315 CCAGTATCGCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1338 TGCGGGATTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1341 ACCACCGTAACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1342 AGAATAGCGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1344 ACATGTCACGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1345 GGCGAACTGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1347 GCAAGTGTGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1348 CCTGGAATTAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1374 CGGACTCGTTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1376 TTCTGGTCTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1377 CACGGTCCTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1378 GTCCTGACACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1379 CACCGTGACACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1380 GATTTAGAGGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1384 ACGGTTTCTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1386 TCACGAGTCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1389 ATAGGTGTGCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1390 CATGTCTTCCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1392 ATACGGGTTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1395 ACTTACGCCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1399 TTGGTGCCTGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1400 GACGGAACAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1402 TTAGACTCGGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1403 CGTACCAGATCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1404 AGGTGGTGGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1405 AGACGTTGCTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1407 CTGGTCTTACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1409 ACCCTATTGCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1410 TGGAAACCATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1520 ATCAGCCAGCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1525 TTACACAAAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1526 TTCTCGGTTCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1527 ACACATAAGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1529 ACGACGCATTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1532 AAGGAGTGCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1533 TCGTAAGCCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1535 TGACGCCTCCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1536 CCAAACTCGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1540 AGCCTCATGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1541 TGAATCGAAGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1542 TTGACACACGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1543 AAGTATCCTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1545 TTACGTGGCGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1546 TGTACGGATAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1547 CTTAGCTACTCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1548 GGAACGACGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1550 GTGACGTTAGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1552 CTGGTGCTGAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1553 CACGTTTATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1554 GTCTGTTGAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1555 TCAGACCAACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1557 TCGTCGCCAAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1560 GGTCGTGTCTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1561 GTGAGGGCAAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1565 CCACGGTACTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1566 CAAGCGTTGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1568 CGTGGGCTCATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1573 GAGTCTTGGTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1576 GCCCAAGTTCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1579 GACTTATGCCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1580 TGAGTTCGGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1582 GACAGAGGTGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1584 GAAACGGAAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1585 AGTGATGTGACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1587 AACATGCATGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1588 CGTCGTCTAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1590 TCGGTCCATAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1591 AATCAACTAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1597 CAATGCCTCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1598 TGTCAGCTGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1599 CATTTCGCACTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1601 CCTTCTGTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1608 TCAATGACCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1609 GTCGCTTGCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1610 CCAATGATAAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1611 ATCGAATCGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1612 CGGATTGCTGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1613 GTTAATGGCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1614 CTCGTTTCAGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1615 AATATCGGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1616 CTCTCATATGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1617 CTTGCATACCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1620 GTATGGAGCTAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1626 CTAGCAGTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1627 ACGCTGTCGGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1631 TCTACCACGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1632 CTATCGGAAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1635 CTTCCGCAGACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1636 GGTACTGTACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1637 TCTGGGCATTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1639 GTGCACGATAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1640 TAGTGCATTCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1641 TTAAACCGCGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1642 TCAGGACGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1644 GCGAACCTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1645 GAATATACCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1647 GAAAGGTGAGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1648 GGTCTAGGTCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1649 GCACTATACGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1926 GCTACTGGTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1927 GCTCCACAACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1928 GAGTTTACGGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1929 ACGTGAGGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1931 GTATAGTCCGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1932 TACTGCCAGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1933 TATAGGCTCCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1934 CGTCACTCCAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1935 CAAATGGTCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1936 TGAGACCCTACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1938 CTGCAGTAAGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1941 GTGTATCGCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1942 AGGGAAAGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1944 GTTATGACGGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP diff --git a/test_data_studies/studies/1288/prep_template_1288.txt b/test_data_studies/studies/1288/prep_template_1288.txt index 86e909da5..720cef752 100644 --- a/test_data_studies/studies/1288/prep_template_1288.txt +++ b/test_data_studies/studies/1288/prep_template_1288.txt @@ -1,1507 +1,1507 @@ sample_name barcodesequence center_name experiment_design_description center_project_name KEY_SEQ library_construction_protocol LINKER platform illumina_technology linkerprimersequence REGION RUN_CENTER RUN_DATE run_prefix SAMPLE_CENTER STUDY_CENTER samp_size sequencing_meth target_gene target_subfragment pcr_primers emp_status -CB01July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB02Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB03June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB05Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB05July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB07June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB08July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB10Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB10June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB12July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB12June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB14July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB14June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB17June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB19July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB20Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB21June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB24June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB24May05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB27May05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB28July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB28June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB30Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CB31May05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -CBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGGCGATCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGGCGATCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTAACGAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTAACGAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGACTCAGACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGACTCAGACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGTGTCGGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGTGTCGGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACACGACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACACGACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCTGGACGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCTGGACGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCTACCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCTACCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGTGCTAATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGTGCTAATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCCTATGAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCCTATGAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGCCGCATATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGCCGCATATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTAGAGCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTAGAGCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGTCATGCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGTCATGCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGTCTCAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGTCTCAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTGTACTCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTGTACTCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACCTGCGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACCTGCGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCGCTCACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCGCTCACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGGATAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGGATAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCAGGACGTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCAGGACGTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCAGCTATTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCAGCTATTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTGGCACTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTGGCACTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACTGAAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACTGAAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCTTCTGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCTTCTGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTTGTCGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTTGTCGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -FBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKE28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH07June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH07June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH15June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH15June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH30July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -HKH30July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA03aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATCTTCTGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA03aug09H.R1.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGTGTCGGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA03aug09H.R2.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGACACGACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA03jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA03jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA06jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA06jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGACTCAGACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA09jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA09jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGTAGAGCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA13aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACACCTGCGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA13aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCTGGACGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA14jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTGTACTCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA14jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTCTACCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA16jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA16jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA19aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTCGGATAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA19aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCCTATGAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA20jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCAGGACGTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA20jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AAGACAGCTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA24aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTACTGAAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA24aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGGCGATCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA24jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA24jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA28jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTGGCACTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA28jul09H.R1.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGCCGCATATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA28jul09H.R2.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCGTCATGCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA30jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA30jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA30may09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MA30may09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01Nov08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE01Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE03June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE05Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCGAATCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCGAATCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE05Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE06June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE08Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE08July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE08Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE09Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE12Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE13June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE15Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATGGAGCTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATGGAGCTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE17July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE17July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE19Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE19June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE20Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE22May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATACCGTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATACCGTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE25Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE26July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE29July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAE30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01Nov08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATGGAGCTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH04July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCCTGTGCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCCTGTGCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH05Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH08July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH08Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH09June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH09Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH11July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH12Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH13June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH15Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH15July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTGACAATAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTGACAATAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH19June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH20June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH20June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH20Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH21Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH22July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGTATCTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGTATCTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH24June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH26July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH26May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH28Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH29July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -MAH30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB01may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB01may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB01nov08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB01nov08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB02jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB02jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB03jul08E.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB03jul08E.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB03jul08H.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB03jul08H.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB03may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB03may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB03oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB04aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTTCGCAGCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB04aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTATGAGTCCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB04may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB04may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB04oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB04oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB05jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB05jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB05may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB05may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB06apr08D1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB06apr08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB06apr08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB07jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGACTGCGTTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB07jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGCTTGTGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB09jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB09jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB09oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB09oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB09sep08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB09sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB10jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTTAGATGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB10jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGAGACGCGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB11aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCATGCTCCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB11aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCATACAGCCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB11jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACCTGTAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB11jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATATCAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB12jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTGCGTTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB12jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AAGAGTCTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB13jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATGTTCGCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB13jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAGTGACCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB14aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATACGATACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB14aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGGAGAGACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB14jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCAGCTGACTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB14jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACACGACTATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB15jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB15jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB15sep08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB15sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB16jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTACACTGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB16jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCGCTAGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB17jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB17jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB17jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB17jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB18aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB18aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB18jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCAAGCATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB18jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGAGTCATTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB18oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB18oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB19jul07H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB19jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB20jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGCATACTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB20jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGTACATGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB21aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCGATCCACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB21jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTCATGTGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB21jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGACAGTAGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB21may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB21may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB21sep08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB21sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB22jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGTACTCTCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB22jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCTAATCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB23jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATATGTGCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB23jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTACTCCACGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB23jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB23jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB23may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB23may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB23oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB24jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACAGGTAGAGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB24jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGACTTCAGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB24mar08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB24mar08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB26aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB26aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB26jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB26jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB27sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB28jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGGCTAGCAGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB28jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTAGCGTTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB28may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB28may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB29apr08D2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB29apr08D3.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB29apr08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB29apr08H.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB29apr08H.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30apr08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30apr08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTCGTGTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCGTATCTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB30may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACTCTGCTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAAGTACTGCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31jun08E.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31jun08E.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31jun08H.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSB31jun08H.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE06Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE06Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE11June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE11June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE30May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE30May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBE31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH02June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH02June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH06May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH06May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH19June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH19June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH24July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH24July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH29June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH29June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH29May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH29May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSBH31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -NSb23oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB02aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB02aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB02jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB02jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB07jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB07jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB08jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB08jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB12aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB12aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB13jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB13jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB15jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB15jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB17aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB21jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB21jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB23jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB23jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB24aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB24aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB27jul09E.R1.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB27jul09E.R2.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB27jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB29jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB29jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB29may09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSB29may09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE03July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE04Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE04Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE05June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE07Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE07July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE09Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE10July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE10Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE11Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE14Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE14July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE19June07.R1.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE19June07.R1.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE19June07.R2.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCTCACGTGTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE19June07.R2.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCTCACGTGTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE21Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE21July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE21Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE23May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE26Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE27Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE30Apr08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBE31July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH03July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH04Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH04Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH06June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH06June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH07Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH07July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCTCTCACTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCTCTCACTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH09June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH09Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH10July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH10Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH11Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH14Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH14July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH14June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH14June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH17June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH18Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH21Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH21July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH21Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH23June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH24July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH26June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH27July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH27July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH27Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH28July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH29July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH29July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH30Apr08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH30June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH30Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH30Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH31Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -SSBH31July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTGCGTTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTGCGTTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE04Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AAGAGTCTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AAGAGTCTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATGTTCGCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATGTTCGCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCAGCTGACTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCAGCTGACTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACACTGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACACTGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTTAGATGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTTAGATGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCAAGCATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCAAGCATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGCATACTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGCATACTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCATGTGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCATGTGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTACTCTCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTACTCTCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE17Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATATGTGCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATATGTGCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE19Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE21Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE21Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE25Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE25Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGACGCGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGACGCGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATATCAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATATCAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGCTTGTGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGCTTGTGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE28Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCGACGAAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE28Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCGACGAAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE290ct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE290ct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE30April08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE30April08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH01July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH01July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH06June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH06June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGTGACCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGTGACCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACGACTATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACGACTATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH11July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH11July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGCTAGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGCTAGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13Jan07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACTGCGTTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13Jan07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACTGCGTTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAGTCATTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAGTCATTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGTACATGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGTACATGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGACAGTAGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGACAGTAGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH17Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH17Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCTAATCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCTAATCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH17Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTACTCCACGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTACTCCACGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH19Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH21JJune07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH21JJune07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH22July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH22July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH25July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH25July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -TBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGATAGCACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGATAGCACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATAATGTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATAATGTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAATGTAGACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAATGTAGACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACATCAGGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACATCAGGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -WSBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to Ê1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB01July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB02Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB03June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB05Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB05July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB07June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB08July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB10Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB10June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB12July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB12June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB14July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB14June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB17June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB19July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB20Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB21June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB24June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB24May05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB27May05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB28July05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB28June05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB30Aug05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CB31May05.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +CBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGGCGATCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGGCGATCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTAACGAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTAACGAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGACTCAGACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGACTCAGACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGTGTCGGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGTGTCGGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACACGACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACACGACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCTGGACGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCTGGACGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCTACCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCTACCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGTGCTAATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGTGCTAATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCCTATGAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCCTATGAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGCCGCATATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGCCGCATATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTAGAGCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTAGAGCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGTCATGCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGTCATGCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH05June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGTCTCAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGTCTCAGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH07June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTGTACTCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTGTACTCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACCTGCGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACCTGCGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCGCTCACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCGCTCACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGGATAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGGATAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCAGGACGTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCAGGACGTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCAGCTATTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCAGCTATTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTGGCACTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTGGCACTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACTGAAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACTGAAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCTTCTGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCTTCTGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH28May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTTGTCGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTTGTCGTGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +FBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKE28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH01Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH04July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH06July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH07June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH07June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH08Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH09July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH10Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH12July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH15June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH15June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH17July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH20July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH20June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH22Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH23July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH24Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH26July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH26June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH28June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH30July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +HKH30July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA03aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATCTTCTGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA03aug09H.R1.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGTGTCGGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA03aug09H.R2.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGACACGACATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA03jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA03jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA06jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA06jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGACTCAGACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA09jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA09jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGTAGAGCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA13aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACACCTGCGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA13aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCTGGACGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA14jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTGTACTCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA14jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTCTACCACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA16jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA16jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA19aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTCGGATAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA19aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCCTATGAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA20jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCAGGACGTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA20jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AAGACAGCTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA24aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTACTGAAGATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA24aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGGCGATCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA24jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA24jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA28jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTGGCACTATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA28jul09H.R1.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGCCGCATATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA28jul09H.R2.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCGTCATGCATC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA30jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA30jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA30may09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MA30may09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01Nov08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE01Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE03June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE05Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCGAATCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCGAATCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE05Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE06June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE08Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE08July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE08Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE09Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE12Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE13June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE15Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATGGAGCTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATGGAGCTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE17July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE17July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE19Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE19June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE20Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE22May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATACCGTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATACCGTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE25Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE26July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE29July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAE30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01Nov08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATGGAGCTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH04July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH04July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCCTGTGCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCCTGTGCGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH05Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH06July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH08Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH08July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH08Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH09July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH09June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH09Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH10Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH11July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH11Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH12Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH13June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH15Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH15July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTGACAATAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH15Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTGACAATAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH18Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH19June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH20July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH20June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH20June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH20Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH21Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH22Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH22July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH23July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGTATCTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH23Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGTATCTGAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH24Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH24June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH26July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH26July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH26June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH26May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH28June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH28Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH29July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +MAH30July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB01may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB01may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB01nov08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB01nov08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB02jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB02jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB03jul08E.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB03jul08E.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB03jul08H.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB03jul08H.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB03may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB03may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB03oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB04aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTTCGCAGCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB04aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTATGAGTCCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB04may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB04may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB04oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB04oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB05jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB05jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB05may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB05may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB06apr08D1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB06apr08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB06apr08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB07jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGACTGCGTTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB07jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGCTTGTGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB09jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB09jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB09oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB09oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB09sep08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB09sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB10jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTTAGATGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB10jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGAGACGCGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB11aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCATGCTCCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB11aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCATACAGCCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB11jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACCTGTAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB11jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATATCAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB12jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTGCGTTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB12jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AAGAGTCTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB13jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATGTTCGCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB13jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTAGTGACCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB14aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATACGATACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB14aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGGAGAGACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB14jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCAGCTGACTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB14jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACACGACTATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB15jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB15jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB15sep08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB15sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB16jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTACACTGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB16jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCGCTAGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB17jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB17jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB17jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB17jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB18aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB18aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB18jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCAAGCATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB18jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGAGTCATTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB18oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB18oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB19jul07H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB19jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB20jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGCATACTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB20jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGTACATGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB21aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCGATCCACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB21jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTCATGTGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB21jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGACAGTAGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB21may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB21may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB21sep08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB21sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB22jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGTACTCTCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB22jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCTAATCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB23jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATATGTGCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB23jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTACTCCACGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB23jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB23jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB23may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB23may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB23oct08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB24jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACAGGTAGAGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB24jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGACTTCAGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB24mar08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB24mar08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB26aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB26aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB26jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB26jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB27sep08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB28jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGGCTAGCAGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB28jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTAGCGTTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB28may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB28may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB29apr08D2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB29apr08D3.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB29apr08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB29apr08H.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB29apr08H.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30apr08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30apr08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTCGTGTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCGTATCTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30jun08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30jun08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30may08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB30may08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31aug08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31aug08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31jul08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACTCTGCTCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31jul08H.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAAGTACTGCAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31jun08E.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31jun08E.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31jun08H.R1.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSB31jun08H.R2.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGATGAGCCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE06Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE06Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GGCTGCATACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE11June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE11June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAACTAGACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACACTGAAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCAAGCTGTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCGGCATCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE30May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE30May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBE31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCAGCCAGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH02Aug09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGAGCTACTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH02June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH02June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH03July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH05June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH06May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH06May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH07July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH08June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH08June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH10July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH11June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH12June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH13July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH15June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH17July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCATGACAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH19June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH19June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH21July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACGTATTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH23June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH24July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH24July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCATTCTCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH25Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH26June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH27July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGGCATCTGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH29June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH29June09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH29May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH29May07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH29May09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSBH31July09.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGAGAATGCTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +NSb23oct08E.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB02aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGAACTAGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB02aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGTGCAACGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB02jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATACGCATCAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB02jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TATCGACACAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB07jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTATCTCCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB07jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCAGTAACTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB08jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTCATCATGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB08jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGATACAGTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB12aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGACGTTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB12aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTGGTCTAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB13jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGCCGATGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB13jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCGTAGTGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB15jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGATCACTCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB15jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACAGTAGCTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB17aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATTGAGTGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB21jul09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACTGACTCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB21jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATAGCTCGGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB23jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGCGAGACTTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB23jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACTCATCATTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB24aug09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGATTCGAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB24aug09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGATGATCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB27jul09E.R1.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGCGATATCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB27jul09E.R2.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAAGATGCAGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB27jul09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCTACTGCGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB29jun09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATTCGAGTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB29jun09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTCAACGCTGTC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB29may09E.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTCTACGAACAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSB29may09H.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGATGTGCTAAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE03July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GGAGCTCTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE04Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE04Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE05June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE07Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE07July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCGCAATAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGTCGATCTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE09Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE10July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE10Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE11Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE14Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE14July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE19June07.R1.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE19June07.R1.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE19June07.R2.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCTCACGTGTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE19June07.R2.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCTCACGTGTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE21Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE21July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CACACGCCTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE21Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TAGAGCTGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGATCGAACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGTGCGTGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE23May08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGAGCATCCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE26Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTGAGCTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTCTCGATCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE27Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTAGTAGACCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE30Apr08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE30Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACGATGAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBE31July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ATCGTCCGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH01Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AAGTCGACACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH03July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH03July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGAAGACGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH04Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGTGTAGTAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH04Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGTCGTGCACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH05July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH06June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH06June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTTCTCTCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH07Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATAGCACCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH07Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACCAGCTCAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH07July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGAGTGAGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCTCTCACTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH07Nov07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCTCTCACTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH09Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGATGGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH09June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH09Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCACTACGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH10July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTATGGAGCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH10Oct08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGCACGATAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH10Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGCGTATCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH11Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GCAGTCTAAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH11July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGATCGCCTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH13July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCACTGGCATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH14Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGAATGAGTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH14July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CAGTAGCGATAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH14June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH14June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH15Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCTCACAGAAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH16July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGACTAGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH17June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence AGCGTCTGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH17Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH18Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH18July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCGCTACGTGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH19July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH19June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACCGTGACACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH21Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH21Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH21July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGTGATCGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH21Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGTGCACGCCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH22June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GACGCACTAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH22Oct07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCTGATAGTTG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH23Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGGAGTCTCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH23June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH24July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH24July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACTACTGAGGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH24Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH25June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGACGTAGAACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH26June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGACGAGATTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH27Aug07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGCCAGTGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH27July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH27July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH27June07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH27Sept08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence GTCGCCGTACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH28July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CCACAGATCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH29July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH29July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAGTTGTACGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH30Apr08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence ACGCATCGCACT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH30June08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CGCCACGTGTAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH30Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH30Sept07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACGGTGACAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH31Aug08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence CTGCTCAGGCAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH31July07.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TATGCCAGAGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +SSBH31July08.McMahon.Pool.3.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.3.1.sequence TCCTCGAGCGAT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-3-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_3_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTAGCGTGCGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTGCGTTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTGCGTTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCATCGAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCAGTACTAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGCGCAACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE04Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTACGGCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCCATGAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTGGTGCATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AAGAGTCTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AAGAGTCTCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGAGAGACAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGACACCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTACGCACAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AATAGCATGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATGTTCGCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATGTTCGCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACACTCACCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCTGACATCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCAGCTGACTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCAGCTGACTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAACGATCATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACACTGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACACTGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTTAGATGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTTAGATGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATGAACAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGTCTAGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATAGCACTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCAAGCATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCAAGCATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGACTGCAGCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGCATACTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGCATACTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTCGCAGATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCATGTGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCATGTGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTACTCTCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTACTCTCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE17Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCTACAGACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGCGCTGAATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTGACGACATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATATGTGCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATATGTGCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AACACTCGATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE19Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGGCTGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE20Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGCCGACTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TAGCAGTTGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE21Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE21Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACATCGTTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE21June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCTCAGTCGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGAGTCACGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAAGATCTATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAGCAGTCTTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCATCGTCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTGTCGATTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE25Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE25Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGACGCGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGACGCGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTATCTGCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATATCAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATATCAGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CAAGCTAGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATCCGTCTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGCTTGTGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGCTTGTGTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE28Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCGACGAAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE28Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCGACGAAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE290ct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE290ct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTCAGCTGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTAGACATGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTCTAGAGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE30April08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE30April08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGACTCTGCGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAACATGTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH01Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH01July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH01July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH01June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CATCGACGAGTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH01Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTACATAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH02Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCACGAGAGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH02Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH02Feb08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH02July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGGAGAGACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH03Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTAAGTGATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH03June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATCCTCATGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GTGTGCTAACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGACGATCCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH04Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05Jan08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGTAACTCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH05Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTAAGACGTCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH06Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCTGATCACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH06July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CGGATCTAGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH06June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH06June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH06Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH07July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCACGTTCTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH07Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATGAATATCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCGACATCTCTT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCTATGTATGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATAGACACTCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGTGACCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH09Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGTGACCTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH09Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACTGAGTACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH09Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAGCATTACATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACGACTATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACGACTATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10Oct08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH11Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CACGTGACATGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH11July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH11July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGCTAGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH11Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGCTAGATAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH12Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH12Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH12July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATAATGCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13Jan07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGACTGCGTTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13Jan07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGACTGCGTTAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGAGTGGTCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATACAGCATACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGCTATCGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH13Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGGTCGCATCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAGTCATTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH14Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAGTCATTGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH15June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACGAGACTGATT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH15June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGTACATGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH15Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGTACATGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCGTTCTAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGACAGTAGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH16Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGACAGTAGGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTAGACTTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH17Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH17Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCTAATCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH17Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCTAATCGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH17Oct05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATTCTCTCACGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCTACAACTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH18Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATGTATGTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH18Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCTCTAGTAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH18July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGTGCTGCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTACTCCACGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH18Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTACTCCACGAG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH19Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACATAAGTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH19June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH19Nov05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ACCACACGTAGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH20July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GACCACTGCTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH20Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CCAGTATCGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH20Sept08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH21JJune07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH21JJune07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTGTAGCCATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH21July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TTAGTCGTGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH22July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH22July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH22June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GCCATAGTGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH22May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TATACCGCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACAGACAATG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH23June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTACATCGCCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH24Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence CTAGTCGCTGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH24Aug09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTATGCGAACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH24June08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH24Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACGATCATCTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH25July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGATGTACACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH25July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH25July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH25Sept05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCCAGATAGCGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH26May08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH27July05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence ATGAAGCACTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTCGATAAGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH27July09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GAGAGATCGACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GGAGAGATCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29July08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29June05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TGTGGCTCGTGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACAGTTACGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29June09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence GATAACTGTACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29May09.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence AGTCTGTCTGCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH29Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCATACACTGG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH30Aug05.McMahon.Pool.4.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.4.1.sequence TCTGATCGAGGT ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-4-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_4_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.2.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_2_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH31Aug08.McMahon.Pool.2.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.8.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-2-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_8_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACTGAGCCTCG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +TBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GACATTGTCACG ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TAGCGACCTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTGCGTATACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATACACGCACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AATAGTCGTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGATAGCACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGATAGCACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGTGAATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CCTACATGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCACTCTTGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE11June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTCGATGTAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGTCTAGAGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAACAGCTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGGCACTATCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAACTGTCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGACGTTGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACACTATGAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGAGGAGTCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTGTGACGTCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATAATGTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE22Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATAATGTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCTGGCTACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGCGTCAGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCAGTACGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATGTGTGTAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGCGGATATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGATACACTGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AGCCAGTCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTGTATCGCCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGAGCAACATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBE31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTCCTACACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH02Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ATCCGCAGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH03July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTGTCAGTGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH05July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACGCTACGACC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH07Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACGATATGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CAATGTAGACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH08Nov07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CAATGTAGACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH09Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CTAGCTATGGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH10Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCGTAGAGAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH11July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTATGCCGTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH13July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CACGCAGTCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH16July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGAAGCATCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH16Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGTCGATTGCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH17Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CGCTATCCAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH19July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence AACTCGCGCTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH19June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGTCTCGCAAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH21Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GAAGACAGCGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH22June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATCTGCGATCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH23Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TTGACACACGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH24July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCTAGACACTAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH25June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GATGTCATAGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACATCAGGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH25Sept07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACATCAGGTCAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH27Aug07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence ACTCCGATAGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH27July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TCGCATGGATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH27June07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GTACATGTCGCC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH29July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TACAGCGCATAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH30Oct07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence TGATAGGTACAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH31July07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence CATCGCGTTGAC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.1.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_1_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +WSBH31May07.McMahon.Pool.1.and.1percentPhiX.110310.HWUSI.EAS552R.0357.s.7.1.sequence GCATACTACAGC ANL Samples taken from temperate bog lakes Temperate Bog Lakes unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 3/10/11 McMahon-Pool-1-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_7_1_sequence ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP From 1363d9bc41253cdff265e9c0afc6eac81c863240 Mon Sep 17 00:00:00 2001 From: Antonio Gonzalez Date: Thu, 17 Aug 2017 09:51:03 -0600 Subject: [PATCH 4/6] =?UTF-8?q?removing=20=C3=93?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- .../studies/1235/prep_template_1235.txt | 536 +++++++++--------- 1 file changed, 268 insertions(+), 268 deletions(-) diff --git a/test_data_studies/studies/1235/prep_template_1235.txt b/test_data_studies/studies/1235/prep_template_1235.txt index 78d5e459f..355ed20f0 100644 --- a/test_data_studies/studies/1235/prep_template_1235.txt +++ b/test_data_studies/studies/1235/prep_template_1235.txt @@ -1,269 +1,269 @@ sample_name barcodesequence center_name experiment_design_description center_project_name KEY_SEQ library_construction_protocol LINKER platform illumina_technology linkerprimersequence REGION RUN_CENTER RUN_DATE run_prefix SAMPLE_CENTER STUDY_CENTER samp_size sequencing_meth target_gene target_subfragment pcr_primers emp_status -sp0192 GATCAACCCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0193 CGTCCGTATGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0195 CTAAGACGTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0200 GAAGAGGGTTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0310 ACACAGTCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0314 GCTTCCAGACAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0317 TTCACCTGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0325 ATTATACGGCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0329 GCGCCGAATCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0333 ATAAAGAGGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0339 ATTCAGATGGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0340 GACTCAACCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0341 CGTATAAATGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0342 CAACTAGACTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0343 AACCTCGGATAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0344 TGCCGCCGTAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0345 GCCTCGTACTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0346 CACGAGCTACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0347 CGCATACGACCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0349 GTGCTTGTGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0350 ACCAACAGATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0351 CATTTGACGACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0352 GTGGCCTACTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0353 TCTCGATAAGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0354 TTCCCTTCTCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0355 AAGTGAAGCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0368 GTAGTGTCAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0370 CATCTGGGCAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0371 AACCCAGATGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0372 AATACAGACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0373 GTCGGAAATTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0374 TCTAACGAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0375 ACTCGGCCAACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0376 AGTGCCCTTGGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0379 TGTCCGTGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0380 GTTGGTTGGCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0381 GGAAGAAGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0382 ACGTAACCACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0383 CACCTGTAGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0384 TTCCACACGTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0701 GGCTCAGATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0702 AGTCCGAGTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0703 GACTCTGCTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0705 TGGTTCATCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0706 ATGAATGCGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0714 CAGAGCTAATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0715 ACTAGTTGGACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0721 AGTAGGAGGCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0722 ATGCGAGACTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0724 AGGTCATCTTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0727 ACACTTCGGCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0729 GTGCTGCGCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0731 TGCTGTGACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0733 GTCCAGCTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0734 TAGTAGCACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0736 CACGTACACGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0738 AGTAGACTTACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0739 CCGCGATTTCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0740 ACCTCCCGGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0742 AGCACTTTGAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0743 CGATTAGGAATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0747 ATTCCTCTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0752 GCTATTCCTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0753 CTCAAGTCAAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0754 CACTGAGTACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0755 TCGTTTCTTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0756 GGATGCAGGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0757 TTGCAAGTACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0758 GCACTTCATTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0759 TTGCCCTTTGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0767 TTGTTACGTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0768 AATGTCACCAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp0769 GTTCTGCTTGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1000 ACGTCTCAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1001 ACACCGCACAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1004 ACTCATCTTCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1005 ACCCGGATTTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1006 TTATCCAGTCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1007 CGCTTGTGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1010 TACGCCCATCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1011 GTAGCACTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1012 AATCTTGCGCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1013 GCATGCATCCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1014 TAGACACCGTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1015 GAAACTCCTAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1016 GACCGTCAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1017 GCCTGCAGTACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1018 AGACAAGCTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1019 CTTGGTAGTGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1020 TCCTAGGTCCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1021 TCCAGGGCTATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1023 TTATGTACGGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1026 ATCGGGCTTAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1027 GAGATACAGTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1028 TCCGCAACCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1029 TCCTCACTATCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1030 AAGATCGTACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1031 TGGAGAGGAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1032 TTGGACGTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1033 ATCGATCCACAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1034 TTGGAACGGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1035 GATCTAATCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1036 GCGTAGAGAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1037 TCACTTGGTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1038 GTCTCCTCCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1039 GGAAATCCCATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1083 CTCCAGGTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1087 TAAACGCGACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1089 ATCCCAGCATGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1098 CCTCGGGTACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1100 CAGGATTCGTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1140 CAAACTGCGTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1142 CTTTAGCGCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1144 GACCGATAGGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1145 CAAGTCGAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1147 CGGCACTATCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1149 CTCGGTCAACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1151 ACACACCCTGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1155 TTAAGCGCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1157 AAGCGTACATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1158 TCGCGCAACTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1160 TCCGTTCGTTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1163 ATGTTTAGACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1164 GGCACACCCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1165 ATTCCCAGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1261 GCAGCCATATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1268 GAATGACGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1271 CATCGGATCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1272 GTCAGCCGTTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1273 ACCTAGCTAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1274 ACGCCTTTCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1275 GTCCACTTGGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1276 CACAAAGCGATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1277 GTTACAGTTGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1278 GGACTCAACTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1279 GAAGATCTATCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1280 CCTTTCACCTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1281 TCTCGCACTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1282 GGACCGCTTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1284 GAATCCTCACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1286 CGCGTCAAACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1287 CATGTTGGAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1288 GTCAAGACCTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1294 CAGCCTGCAAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1295 GTCTCTGAAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1298 CCACTTGAGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1299 ATGGGACCTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1300 AGAATCCACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1304 ATAAGGTCGCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1307 AGTACCTAAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1308 CGAGATAGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1309 GCTTCTCTCACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1310 GTACCTAGCCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1312 CAGTTCGAGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1314 TCAAGCAATACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1315 CCAGTATCGCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1338 TGCGGGATTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1341 ACCACCGTAACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1342 AGAATAGCGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1344 ACATGTCACGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1345 GGCGAACTGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1347 GCAAGTGTGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1348 CCTGGAATTAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1374 CGGACTCGTTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1376 TTCTGGTCTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1377 CACGGTCCTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1378 GTCCTGACACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1379 CACCGTGACACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1380 GATTTAGAGGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1384 ACGGTTTCTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1386 TCACGAGTCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1389 ATAGGTGTGCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1390 CATGTCTTCCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1392 ATACGGGTTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1395 ACTTACGCCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1399 TTGGTGCCTGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1400 GACGGAACAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1402 TTAGACTCGGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1403 CGTACCAGATCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1404 AGGTGGTGGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1405 AGACGTTGCTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1407 CTGGTCTTACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1409 ACCCTATTGCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1410 TGGAAACCATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1520 ATCAGCCAGCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1525 TTACACAAAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1526 TTCTCGGTTCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1527 ACACATAAGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1529 ACGACGCATTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1532 AAGGAGTGCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1533 TCGTAAGCCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1535 TGACGCCTCCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1536 CCAAACTCGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1540 AGCCTCATGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1541 TGAATCGAAGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1542 TTGACACACGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1543 AAGTATCCTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1545 TTACGTGGCGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1546 TGTACGGATAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1547 CTTAGCTACTCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1548 GGAACGACGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1550 GTGACGTTAGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1552 CTGGTGCTGAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1553 CACGTTTATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1554 GTCTGTTGAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1555 TCAGACCAACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1557 TCGTCGCCAAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1560 GGTCGTGTCTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1561 GTGAGGGCAAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1565 CCACGGTACTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1566 CAAGCGTTGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1568 CGTGGGCTCATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1573 GAGTCTTGGTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1576 GCCCAAGTTCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1579 GACTTATGCCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1580 TGAGTTCGGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1582 GACAGAGGTGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1584 GAAACGGAAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1585 AGTGATGTGACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1587 AACATGCATGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1588 CGTCGTCTAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1590 TCGGTCCATAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1591 AATCAACTAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1597 CAATGCCTCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1598 TGTCAGCTGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1599 CATTTCGCACTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1601 CCTTCTGTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1608 TCAATGACCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1609 GTCGCTTGCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1610 CCAATGATAAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1611 ATCGAATCGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1612 CGGATTGCTGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1613 GTTAATGGCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1614 CTCGTTTCAGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1615 AATATCGGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1616 CTCTCATATGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1617 CTTGCATACCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1620 GTATGGAGCTAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1626 CTAGCAGTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1627 ACGCTGTCGGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1631 TCTACCACGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1632 CTATCGGAAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1635 CTTCCGCAGACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1636 GGTACTGTACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1637 TCTGGGCATTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1639 GTGCACGATAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1640 TAGTGCATTCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1641 TTAAACCGCGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1642 TCAGGACGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1644 GCGAACCTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1645 GAATATACCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1647 GAAAGGTGAGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1648 GGTCTAGGTCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1649 GCACTATACGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1926 GCTACTGGTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1927 GCTCCACAACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1928 GAGTTTACGGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1929 ACGTGAGGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1931 GTATAGTCCGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1932 TACTGCCAGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1933 TATAGGCTCCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1934 CGTCACTCCAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1935 CAAATGGTCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1936 TGAGACCCTACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1938 CTGCAGTAAGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1941 GTGTATCGCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1942 AGGGAAAGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -sp1944 GTTATGACGGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommendedÓ regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0192 GATCAACCCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0193 CGTCCGTATGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0195 CTAAGACGTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0200 GAAGAGGGTTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0310 ACACAGTCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0314 GCTTCCAGACAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0317 TTCACCTGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0325 ATTATACGGCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0329 GCGCCGAATCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0333 ATAAAGAGGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0339 ATTCAGATGGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0340 GACTCAACCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0341 CGTATAAATGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0342 CAACTAGACTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0343 AACCTCGGATAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0344 TGCCGCCGTAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0345 GCCTCGTACTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0346 CACGAGCTACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0347 CGCATACGACCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0349 GTGCTTGTGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0350 ACCAACAGATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0351 CATTTGACGACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0352 GTGGCCTACTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0353 TCTCGATAAGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0354 TTCCCTTCTCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0355 AAGTGAAGCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0368 GTAGTGTCAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0370 CATCTGGGCAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0371 AACCCAGATGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0372 AATACAGACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0373 GTCGGAAATTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0374 TCTAACGAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0375 ACTCGGCCAACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0376 AGTGCCCTTGGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0379 TGTCCGTGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0380 GTTGGTTGGCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0381 GGAAGAAGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0382 ACGTAACCACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0383 CACCTGTAGTAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0384 TTCCACACGTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0701 GGCTCAGATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0702 AGTCCGAGTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0703 GACTCTGCTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0705 TGGTTCATCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0706 ATGAATGCGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0714 CAGAGCTAATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0715 ACTAGTTGGACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0721 AGTAGGAGGCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0722 ATGCGAGACTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0724 AGGTCATCTTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0727 ACACTTCGGCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0729 GTGCTGCGCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0731 TGCTGTGACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0733 GTCCAGCTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0734 TAGTAGCACCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0736 CACGTACACGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0738 AGTAGACTTACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0739 CCGCGATTTCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0740 ACCTCCCGGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0742 AGCACTTTGAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0743 CGATTAGGAATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0747 ATTCCTCTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0752 GCTATTCCTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0753 CTCAAGTCAAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0754 CACTGAGTACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0755 TCGTTTCTTCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0756 GGATGCAGGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0757 TTGCAAGTACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0758 GCACTTCATTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0759 TTGCCCTTTGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0767 TTGTTACGTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0768 AATGTCACCAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp0769 GTTCTGCTTGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1000 ACGTCTCAGTGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1001 ACACCGCACAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1004 ACTCATCTTCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1005 ACCCGGATTTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1006 TTATCCAGTCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1007 CGCTTGTGTAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1010 TACGCCCATCAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1011 GTAGCACTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1012 AATCTTGCGCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1013 GCATGCATCCCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1014 TAGACACCGTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1015 GAAACTCCTAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1016 GACCGTCAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1017 GCCTGCAGTACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1018 AGACAAGCTTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1019 CTTGGTAGTGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1020 TCCTAGGTCCGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1021 TCCAGGGCTATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1023 TTATGTACGGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1026 ATCGGGCTTAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1027 GAGATACAGTTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1028 TCCGCAACCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1029 TCCTCACTATCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1030 AAGATCGTACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1031 TGGAGAGGAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1032 TTGGACGTCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1033 ATCGATCCACAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1034 TTGGAACGGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1035 GATCTAATCGAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1036 GCGTAGAGAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1037 TCACTTGGTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1038 GTCTCCTCCCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1039 GGAAATCCCATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1083 CTCCAGGTCATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1087 TAAACGCGACTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1089 ATCCCAGCATGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1098 CCTCGGGTACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1100 CAGGATTCGTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1140 CAAACTGCGTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1142 CTTTAGCGCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1144 GACCGATAGGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1145 CAAGTCGAATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1147 CGGCACTATCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1149 CTCGGTCAACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1151 ACACACCCTGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1155 TTAAGCGCCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1157 AAGCGTACATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1158 TCGCGCAACTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1160 TCCGTTCGTTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1163 ATGTTTAGACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1164 GGCACACCCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1165 ATTCCCAGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1261 GCAGCCATATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1268 GAATGACGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1271 CATCGGATCTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1272 GTCAGCCGTTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1273 ACCTAGCTAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1274 ACGCCTTTCTTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1275 GTCCACTTGGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1276 CACAAAGCGATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1277 GTTACAGTTGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1278 GGACTCAACTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1279 GAAGATCTATCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1280 CCTTTCACCTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1281 TCTCGCACTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1282 GGACCGCTTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1284 GAATCCTCACCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1286 CGCGTCAAACTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1287 CATGTTGGAACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1288 GTCAAGACCTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1294 CAGCCTGCAAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1295 GTCTCTGAAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1298 CCACTTGAGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1299 ATGGGACCTTCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1300 AGAATCCACCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1304 ATAAGGTCGCCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1307 AGTACCTAAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1308 CGAGATAGTTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1309 GCTTCTCTCACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1310 GTACCTAGCCTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1312 CAGTTCGAGATA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1314 TCAAGCAATACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1315 CCAGTATCGCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1338 TGCGGGATTCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1341 ACCACCGTAACC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1342 AGAATAGCGCTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1344 ACATGTCACGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1345 GGCGAACTGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1347 GCAAGTGTGAGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1348 CCTGGAATTAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1374 CGGACTCGTTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1376 TTCTGGTCTTGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1377 CACGGTCCTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1378 GTCCTGACACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1379 CACCGTGACACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1380 GATTTAGAGGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1384 ACGGTTTCTGGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1386 TCACGAGTCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1389 ATAGGTGTGCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1390 CATGTCTTCCAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1392 ATACGGGTTCGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1395 ACTTACGCCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1399 TTGGTGCCTGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1400 GACGGAACAGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1402 TTAGACTCGGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1403 CGTACCAGATCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1404 AGGTGGTGGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1405 AGACGTTGCTAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1407 CTGGTCTTACGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1409 ACCCTATTGCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1410 TGGAAACCATTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1520 ATCAGCCAGCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1525 TTACACAAAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1526 TTCTCGGTTCTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1527 ACACATAAGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1529 ACGACGCATTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1532 AAGGAGTGCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1533 TCGTAAGCCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1535 TGACGCCTCCAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1536 CCAAACTCGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1540 AGCCTCATGATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1541 TGAATCGAAGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1542 TTGACACACGAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1543 AAGTATCCTGCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1545 TTACGTGGCGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1546 TGTACGGATAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1547 CTTAGCTACTCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1548 GGAACGACGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1550 GTGACGTTAGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1552 CTGGTGCTGAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1553 CACGTTTATTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1554 GTCTGTTGAGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1555 TCAGACCAACTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1557 TCGTCGCCAAAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1560 GGTCGTGTCTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1561 GTGAGGGCAAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1565 CCACGGTACTTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1566 CAAGCGTTGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1568 CGTGGGCTCATT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1573 GAGTCTTGGTAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1576 GCCCAAGTTCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1579 GACTTATGCCCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1580 TGAGTTCGGTCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1582 GACAGAGGTGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1584 GAAACGGAAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1585 AGTGATGTGACT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1587 AACATGCATGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1588 CGTCGTCTAAGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1590 TCGGTCCATAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1591 AATCAACTAGGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1597 CAATGCCTCACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1598 TGTCAGCTGTCG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1599 CATTTCGCACTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1601 CCTTCTGTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1608 TCAATGACCGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1609 GTCGCTTGCACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1610 CCAATGATAAGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1611 ATCGAATCGAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1612 CGGATTGCTGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1613 GTTAATGGCAGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1614 CTCGTTTCAGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1615 AATATCGGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1616 CTCTCATATGCT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1617 CTTGCATACCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1620 GTATGGAGCTAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1626 CTAGCAGTATGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1627 ACGCTGTCGGTT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1631 TCTACCACGAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1632 CTATCGGAAGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1635 CTTCCGCAGACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1636 GGTACTGTACCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1637 TCTGGGCATTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1639 GTGCACGATAAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1640 TAGTGCATTCGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1641 TTAAACCGCGCC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1642 TCAGGACGTATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1644 GCGAACCTATAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1645 GAATATACCTGG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1647 GAAAGGTGAGAA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1648 GGTCTAGGTCTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1649 GCACTATACGCA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1926 GCTACTGGTATG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1927 GCTCCACAACGT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1928 GAGTTTACGGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1929 ACGTGAGGAACG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1931 GTATAGTCCGTG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1932 TACTGCCAGTGA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1933 TATAGGCTCCGC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1934 CGTCACTCCAAG ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1935 CAAATGGTCGTC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1936 TGAGACCCTACA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1938 CTGCAGTAAGTA ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1941 GTGTATCGCCAC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1942 AGGGAAAGGATC ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +sp1944 GTTATGACGGAT ANL CO2 pressures were increased in multiple mesocosms and samples within each were taken during an elongated period of time. Marine microbial communities unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair ampli_es the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base errorcorrecting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. The three sequencing primers include two for reading in from each end of the amplicon and a third for reading the barcode. Because of technical limitations at the sequencing facility, only part of the barcode was sequenced, so we were unable to exploit the error-correcting properties fully; however, even with partial barcodes we were able to resolve the samples, demonstrating the robustness of the approach. It is important to note that this primer collection allows for sequencing of paired-end reads, but the downstream data analyses are not yet capable of supporting paired-end reads. Our results illustrate interesting and correlated patterns based on analysis of the unpaired reads (i.e., _ and _ diversity evaluations based on the 5_ only and 3_ only reads independently achieve similar results, suggesting that 100 bases in this region of the 16S gene can allow for successful screening and comparison of microbial communities). The reads generated from these PCR primers are both identi_ed as _recommended regions by Liu et al. (15). Polymerase Chain Reaction. Sample preparation was performed similarly to that described by Costello et al. (1). Brie_y, each sample was ampli_ed in triplicate, combined, and cleaned using the MO BIO 96 htp PCR clean up kit. PCR reactions contained 13 _L MO BIO PCR water, 10 _L 5 Prime Hot Master Mix, 0.5 _L each of the forward and reverse primers (10 _M _nal concentration), and 1.0 _L genomic DNA. Reactions were held at 94 C for 3 min to denature the DNA, with ampli_cation proceeding for 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; a _nal extension of 10 min at 72 C was added to ensure complete ampli_cation. Cleaned amplicons were quanti_ed using Picogreen dsDNA reagent in 10 mM Tris buffer (pH 8.0). A composite sample for sequencing was created by combining equimolar ratios of amplicons from the individual samples, followed by gel puri_cation and ethanol precipitation to remove any remaining contaminants and PCR artifacts. GT Illumina HiSeq GTGCCAGCMGCCGCGGTAA unknown ANL 2011 s_5_1_sequences ANL CCME .1,g Sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP From c6c852c2452693eb58e8f0d5c1892a27c14f6f05 Mon Sep 17 00:00:00 2001 From: Antonio Gonzalez Date: Thu, 17 Aug 2017 11:56:36 -0600 Subject: [PATCH 5/6] 🐾 --- qiita_db/metadata_template/util.py | 10 ++++++---- qiita_ware/test/test_dispatchable.py | 8 +++++--- 2 files changed, 11 insertions(+), 7 deletions(-) diff --git a/qiita_db/metadata_template/util.py b/qiita_db/metadata_template/util.py index 3807a1f0a..7228bb49f 100644 --- a/qiita_db/metadata_template/util.py +++ b/qiita_db/metadata_template/util.py @@ -110,16 +110,18 @@ def load_template_to_dataframe(fn, index='sample_name'): for col, block in enumerate(line.split('\t')): tblock = ''.join([c for c in block if c in printable]) if len(block) != len(tblock): - tblock = ''.join([c if c in printable else '♥' + tblock = ''.join([c if c in printable else '🐾' for c in block]) if tblock not in errors: errors[tblock] = [] errors[tblock].append('(%d, %d)' % (row, col)) if bool(errors): raise ValueError( - "There are non valid UTF-8 characters. The errors are " - "shown as ♥: %s" % '; '.join( - ['%s = %s' % (k, ', '.join(v)) + "There are invalid (non UTF-8) characters in your information " + "file. The offending fields and their location (row, column) " + "are listed below, invalid characters are represented using " + "🐾: %s" % '; '.join( + ['"%s" = %s' % (k, ', '.join(v)) for k, v in viewitems(errors)])) if not holdfile: diff --git a/qiita_ware/test/test_dispatchable.py b/qiita_ware/test/test_dispatchable.py index 711838efc..00e3bf0d6 100644 --- a/qiita_ware/test/test_dispatchable.py +++ b/qiita_ware/test/test_dispatchable.py @@ -84,9 +84,11 @@ def test_create_sample_template_nonutf8(self): 'sample_info_utf8_error.txt') obs = create_sample_template(fp, Study(1), False) exp = {'status': 'danger', - 'message': "There are non valid UTF-8 characters. The errors " - "are shown as ♥: ♥collection_timestamp " - "= (0, 13)"} + 'message': 'There are invalid (non UTF-8) characters in your ' + 'information file. The offending fields and their ' + 'location (row, column) are listed below, invalid ' + 'characters are represented using 🐾: ' + '"🐾collection_timestamp" = (0, 13)'} self.assertEqual(obs, exp) def test_update_sample_template(self): From 19ac949c68ca116574996f9267a5b39cbbc18594 Mon Sep 17 00:00:00 2001 From: Antonio Gonzalez Date: Thu, 17 Aug 2017 12:27:52 -0600 Subject: [PATCH 6/6] =?UTF-8?q?=C3=A6?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- test_data_studies/studies/895/prep_template_895.txt | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/test_data_studies/studies/895/prep_template_895.txt b/test_data_studies/studies/895/prep_template_895.txt index df6a77fd6..9266d14d3 100644 --- a/test_data_studies/studies/895/prep_template_895.txt +++ b/test_data_studies/studies/895/prep_template_895.txt @@ -1,7 +1,7 @@ sample_name barcodesequence center_name experiment_design_description center_project_name KEY_SEQ library_construction_protocol LINKER illumina_technology platform linkerprimersequence REGION RUN_CENTER RUN_DATE run_prefix SAMPLE_CENTER STUDY_CENTER samp_size sequencing_meth target_gene target_subfragment pcr_primers emp_status -Puhimau.mat.1 TCGAGCCGATCT ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to æ1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -Puhimau.mat.2 CTCATCATGTTC ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to æ1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -Puhimau.mat.3 CCAGGGACTTCT ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to æ1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -Puhimau.soil.1 GCAATCCTTGCG ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to æ1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -Puhimau.soil.2 CCTGCTTCCTTC ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to æ1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP -Puhimau.soil.3 CAAGGCACAAGG ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to æ1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +Puhimau.mat.1 TCGAGCCGATCT ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +Puhimau.mat.2 CTCATCATGTTC ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +Puhimau.mat.3 CCAGGGACTTCT ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +Puhimau.soil.1 GCAATCCTTGCG ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +Puhimau.soil.2 CCTGCTTCCTTC ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP +Puhimau.soil.3 CAAGGCACAAGG ANL Examination of geothermal sites on Kilauea. One site involves a steaming tumulus that supports a stratified biofilm at about 50 C. The site other involves a forest that was destroyed by sub-surface heating. What exists at present are heated soils and biofilms that develop on steaming tree trunks. The latter include anoxygenic chloroflexi plus a variety of CO oxidizers. Gasser_Kilauea_geothermal unknown This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. GT HiSeq Illumina GTGCCAGCMGCCGCGGTAA 0 ANL 8/14/12 lane5_NoIndex ANL ANL .1,g sequencing by synthesis 16S rRNA V4 FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT EMP