diff --git a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts
index 501a657caf81..fc7a045b831d 100644
--- a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts
+++ b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts
@@ -24,7 +24,6 @@
 
 	aliases {
 		led0 = &ld26;
-		adc0 = &adc0;
 	};
 
 	leds: leds {
@@ -89,89 +88,8 @@
 };
 
 &adc0 {
-	ti,vrefp = <1800>;
 	ti,fifo = <0>;
 	status = "okay";
-
-	channel@0 {
-		reg = <0>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
-
-	channel@1 {
-		reg = <1>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
-
-	channel@2 {
-		reg = <2>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
-
-	channel@3 {
-		reg = <3>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
-
-	channel@4 {
-		reg = <4>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
-
-	channel@5 {
-		reg = <5>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
-
-	channel@6 {
-		reg = <6>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
-
-	channel@7 {
-		reg = <7>;
-		ti,open-delay = <0>;
-		zephyr,gain = "ADC_GAIN_1";
-		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <0>;
-		zephyr,resolution = <12>;
-		zephyr,oversampling = <4>;
-	};
 };
 
 &mbox6 {
diff --git a/samples/drivers/adc/adc_dt/boards/am243x_evm_am2434_r5f0_0.overlay b/samples/drivers/adc/adc_dt/boards/am243x_evm_am2434_r5f0_0.overlay
index 5d8dccf46af8..c82270c8c71c 100644
--- a/samples/drivers/adc/adc_dt/boards/am243x_evm_am2434_r5f0_0.overlay
+++ b/samples/drivers/adc/adc_dt/boards/am243x_evm_am2434_r5f0_0.overlay
@@ -6,7 +6,30 @@
 
 / {
 	zephyr,user {
-		io-channels = <&adc0 0>, <&adc0 1>, <&adc0 2>, <&adc0 3>, <&adc0 4>, <&adc0 5>,
-			      <&adc0 6>, <&adc0 7>;
+		io-channels = <&adc0 0>, <&adc0 7>;
+	};
+};
+
+&adc0 {
+	ti,vrefp = <1800>;
+
+	channel@0 {
+		reg = <0>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@7 {
+		reg = <7>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
 	};
 };
diff --git a/samples/drivers/adc/adc_sequence/boards/am243x_evm_am2434_r5f0_0.overlay b/samples/drivers/adc/adc_sequence/boards/am243x_evm_am2434_r5f0_0.overlay
new file mode 100644
index 000000000000..62ea18e74211
--- /dev/null
+++ b/samples/drivers/adc/adc_sequence/boards/am243x_evm_am2434_r5f0_0.overlay
@@ -0,0 +1,95 @@
+/*
+ * Copyright (c) 2025 Texas Instruments
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/ {
+	aliases {
+		adc0 = &adc0;
+	};
+};
+
+&adc0 {
+	ti,vrefp = <1800>;
+
+	channel@0 {
+		reg = <0>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@1 {
+		reg = <1>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@2 {
+		reg = <2>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@3 {
+		reg = <3>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@4 {
+		reg = <4>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@5 {
+		reg = <5>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@6 {
+		reg = <6>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+
+	channel@7 {
+		reg = <7>;
+		ti,open-delay = <0>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <0>;
+		zephyr,resolution = <12>;
+		zephyr,oversampling = <4>;
+	};
+};